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BIS-Tris

BIS-Tris structure

CAS No.: 6976-37-0

Chemical Name:: BIS-Tris

Synonyms: BIS-TRIS;BIS(2-HYDROXYETHYL)IMINOTRIS(HYDROXYMETHYL)METHANE;2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol;Bis-Iris;2,2-BIS(HY;''BIS-TRIS'';BIS-TRIS >99%;BIS-TRIS >98%;BIS-TRIS BUFFER;BIS-Tris, ULTROL

CBNumber:: CB4304881

Molecular Formula:: C8H19NO5

Molecular Weight:: 209.24

MDL Number:: MFCD00002853

MOL File:: 6976-37-0.mol

MSDS File:: SDS

Last updated:2025-01-27 09:38:02

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BIS-Tris Properties

Melting point	104 °C(lit.)
Boiling point	466.2±40.0 °C(Predicted)
Density	1.346±0.06 g/cm3(Predicted)
vapor pressure	0Pa at 25℃
storage temp.	Inert atmosphere,Room Temperature
solubility	H₂O: 1 M at 20 °C, clear, colorless
form	Solution
color	Yellow to orange
Odor	Odorless
PH Range	5.8 - 7.2
PH	9.0-10.1 (20-25℃, 1M in water)
pka	6.5(at 25℃)
biological source	synthetic
Water Solubility	soluble
Sensitive	Air Sensitive
λmax	λ: 280 nm Amax: 0.15
BRN	2205275
InChIKey	OWMVSZAMULFTJU-UHFFFAOYSA-N
LogP	-2.26 at 20℃
CAS DataBase Reference	6976-37-0(CAS DataBase Reference)
EWG's Food Scores	1
FDA UNII	Q1XC3631CP
EPA Substance Registry System	1,3-Propanediol, 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)- (6976-37-0)
Absorption	cut-off at 270nm in H2O at 1M
UNSPSC Code	12352200
NACRES	NA.25

SAFETY

Risk and Safety Statements

Symbol(GHS)

GHS05

Signal word

Danger

Hazard statements

H318

Precautionary statements

P280-P305+P351+P338+P310

Hazard Codes

Xi,Xn

Risk Statements

36/37/38-21/22-20/21/22

Safety Statements

26-36-36/37/39-22

WGK Germany

10-23

TSCA

Yes

HS Code

29221980

NFPA 704

	1
2		0

BIS-Tris price More Price(91)

Manufacturer	Product number	Product description	CAS number	Packaging	Price	Updated	Buy
Sigma-Aldrich	PHG0004	BIS-TRIS	6976-37-0	1KIT	$1123	2024-03-01	Buy
Sigma-Aldrich	B4429	BIS-TRIS BioPerformance Certified, cell culture tested, suitable for insect cell culture, ≥98.0%	6976-37-0	1kg	$1220	2024-03-01	Buy
Sigma-Aldrich	391335	BIS-Tris, ULTROL Grade - CAS 6976-37-0 - Calbiochem Azwitterionicbufferthatexhibitsonlyaverysmallshiftindissociationcons	6976-37-0	100g	$179	2024-03-01	Buy
Sigma-Aldrich	391335	BIS-Tris, ULTROL Grade - CAS 6976-37-0 - Calbiochem Azwitterionicbufferthatexhibitsonlyaverysmallshiftindissociationcons	6976-37-0	1kg	$898	2024-03-01	Buy
Sigma-Aldrich	14879	BIS-TRIS BioUltra, ≥99.0% (NT)	6976-37-0	100g	$244	2024-03-01	Buy

Product number	Packaging	Price	Buy
PHG0004	1KIT	$1123	Buy
B4429	1kg	$1220	Buy
391335	100g	$179	Buy
391335	1kg	$898	Buy
14879	100g	$244	Buy

BIS-Tris Chemical Properties,Uses,Production

Chemical Properties

White/clear crystalline powder

Uses

Bis Tris is a commonly used biological buffer for very broad usage in biological systems such as diagnostics, electrophoresis and protein based bioprocessing. The pKa of Bis Tris is 6.36 which makes it a good candidate for biological-based formulations.

Bis Tris is used in a wide variety of biological applications including protein separation buffers, cell culture media, electrophoresis separations and diagnostic reagent formulations.

Uses

It is used as a biological buffer, organic synthesize, chemical & pharmaceutical intermediate. It is suitable for fractionation of hemoglobin.

Uses

Bis-Tris, also known as bis-Tris methane, is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a bis(2-hydroxyethyl)amine and Tris family buffer. Bis Tris is a common component of many buffer systems for various types of electrophoresis. Bis-Tris may form a complex with some common metals, such as Cu(II) and Pb(II), and formation constants should be taken into account when using this buffer in a solution containing metal ions. Bis-Tris can be used as a substitute for the highly toxic buffer cacodylate.

Definition

ChEBI: Bis-tris is a buffer substance useful for calibration of glass electrodes and for the preparation of the biochemical and biological buffer solutions; pKa = 6.46 at 25 ℃. It has a role as a buffer. It is functionally related to a member of tris.

Flammability and Explosibility

Not classified

Purification Methods

Crystallise BIS-TRIS from hot 1-butanol and dry it in a vacuum at 25o. N,N-Bis-(2-hydroxyethyl)glycine (BICINE) See in “Amino Acids and Peptides”, Chapter 6.

BIS-Tris Preparation Products And Raw materials

Raw materials

Preparation Products

BIS-Tris Suppliers

Global( 468)Suppliers

Supplier	Tel	Email	Country	ProdList	Advantage
Jiangsu Magic Biotechnology Co., Ltd	+undefined13921770081	SVP01@magicbiotech.cn	China	92	58
Suzhou Genemill Biotechnology Co.,Ltd	+8615862502491	aimeegao@genemill.com	China	39	58
Aladdin Scientific		tp@aladdinsci.com	United States	57505	58
Watson Biotechnology Co.,Ltd	+86-18186686046 +86-18186686046	sales01@watsonbiotech.cn	China	5858	58
SHANGHAI KEAN TECHNOLOGY CO., LTD.	+8613817748580	cooperation@kean-chem.com	China	40066	58
Hebei Chuanghai Biotechnology Co., Ltd	+8617732866630	bess@weibangbio.com	China	18144	58
Hebei Mujin Biotechnology Co.,Ltd	+86 13288715578 +8613288715578	sales@hbmojin.com	China	12825	58
Hebei Yanxi Chemical Co., Ltd.	+8617531153977	allison@yan-xi.com	China	5855	58
Hebei Chuanghai Biotechnology Co,.LTD	+86-13131129325	sales1@chuanghaibio.com	China	5879	58
Henan Fengda Chemical Co., Ltd	+86-371-86557731 +86-13613820652	info@fdachem.com	China	20257	58

Supplier	Advantage
Jiangsu Magic Biotechnology Co., Ltd	58
Suzhou Genemill Biotechnology Co.,Ltd	58
Aladdin Scientific	58
Watson Biotechnology Co.,Ltd	58
SHANGHAI KEAN TECHNOLOGY CO., LTD.	58
Hebei Chuanghai Biotechnology Co., Ltd	58
Hebei Mujin Biotechnology Co.,Ltd	58
Hebei Yanxi Chemical Co., Ltd.	58
Hebei Chuanghai Biotechnology Co,.LTD	58
Henan Fengda Chemical Co., Ltd	58

View Lastest Price from BIS-Tris manufacturers

Update time	Product	Price	Min. Order	Purity	Supply Ability	Manufacturer
2025-03-05	2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol 6976-37-0	US $0.00 / KG	1KG	99%	50000KG/month	Hebei Mujin Biotechnology Co.,Ltd
2025-03-05	BIS-TRIS 6976-37-0	US $0.00-0.00 / kg	1kg	99%	20MT	Watson Biotechnology Co.,Ltd
2025-03-05	Bis-Tris 6976-37-0	US $180.00 / kg	1kg	99%	20ton	Hebei Zhuanglai Chemical Trading Co.,Ltd

2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol
6976-37-0
US $0.00 / KG
99%
Hebei Mujin Biotechnology Co.,Ltd

BIS-TRIS
6976-37-0
US $0.00-0.00 / kg
99%
Watson Biotechnology Co.,Ltd

Bis-Tris
6976-37-0
US $180.00 / kg
99%
Hebei Zhuanglai Chemical Trading Co.,Ltd

BIS-Tris Spectrum

BIS-Tris(6976-37-0)MS BIS-Tris(6976-37-0)¹HNMR BIS-Tris(6976-37-0)IR1 BIS-Tris(6976-37-0)IR2 BIS-Tris(6976-37-0)Raman

6976-37-0(BIS-Tris)Related Search:

4,4'-Methylenedianiline 2-(METHYLSULFONYL)ETHANOL Hydroxyethyl starch Tricine Tris(hydroxymethyl)nitromethane

Hydroxyethyl Cellulose Tris(hydroxymethyl)aminomethane Methyl 2-hydroxyethyl cellulose 2-(2-Aminoethylamino)ethanol BUFFER SOLUTION PH = 6,00 (20 °C) (POTASSIUM DIHYDROGEN PHOSPHATE/SODIUM HYDROXIDE)

Sodium isethionate 2-(ISOPROPYLAMINO)ETHANOL tert-Butylamine Triethylamine Diethanolamine

2-Amino-1,3-propanediol BIS-TRIS-D19 Hydroxymethyl

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2,2-BIS(HYDROXYETHYL)-(IMINOTRIS)-(HYDROXYMETHYL)-METHANE 2-BIS[2-HYDROXYETHYL]AMINO-2-[HYDROXYMETHYL]1,3-PROPANEDIOL 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)-3-propanediol Bis Tris Bis(2-hydroxyethyl)imino-tris(hydroxymethyl)methane Bis Tris, Molecular Biology Grade Bis(2-hydroxyethyl)imino-tris(hydroxymethyl)methane, Molecular Biology Grade Bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane~Bis-TRIS Bis-Tris (2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol) BIS-TRIS BIOULTRA, >= 99.0% (NT) BIS-TRIS, BIOPERFORMANCE CERTIFIED& 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane Bis-tris BIS-Tris, ULTROL Bis-Tris, Cell culture reagent BIS(2-HYDROXYETHYL)IMINO-TRIS(HYDROXYMETHYL)PROPANE BIS(2-HYDROXYETHYL)AMINO-TRIS(HYDROXYMETHYL)METHANE BIS(HYDROXYETHYL)IMINO-TRIS-(HYDROXYMETHYL)-METHANE ''BIS-TRIS'' BIS-TRIS BUFFER 2,2-BIS(HYDROXYMETHYL)-2,2',2''-NITRILOETHANOL 2,2-BIS-(HYDROXYMETHYL)-2,2',2'-NITRILOTRIETHANOL 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol, 99+% 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol, for biochemistry, 99+% Bis[(2-hydroxyethyl)amino]-2-(hydroxymethyl)- 1,3-propanediol 1,3-Propanediol, 2-bis(2-hydroxyethyl)amino-2-(hydroxymethyl)- Bis(2-hydroxyethyl)iminotris(hydroxylmethyl)methane BIS-TRIS,BIOLOGICALBUFFER Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)propane BIS(2-HYDROXYETHYL)-TRIS(HYDROXYMETHYL)METHANE BIS-TRIS (BIS(2-HYDROXYETHYL)AMINO-TRIS(HYDROXYMETHYL)METHANE) BIS(2-HYDROXYETHYL)IMINOTRIS(HYDROXYMETHYL)METHANE(BIS-TRIS) Bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane >98% Bis(2-hydroxyethyl)-imino-tris-(hydroxymethyl)-methane biological buffer bis-tris cell culture tested bis-tris free base bis-tris free base sigmaultra BIS-Tris, ULTROL Grade 2,2-BIS(HYDROXYMETHYL)-2,2',2''-NITRILO- TRIETHANOL, 98+% BIS-TRIS, BIOTECHNOLOGY PERFORMANCE CERT IFIED BIS(2-HYDROXYETHYL)AMINO-TRIS(HYDROXYMET L)-METHAN BIS(2-HYDROXYETHYL)IMINO-TRIS(HYDROXYMETHYL)PROPANE （BIS-TRIS） (2-Hydroxyethyl)iMinotris(hydroxyMethyl)Methane(BIS-TRIS) 2-[bis(2-hydroxyethyl)aMino]-3-(hydroxyMethyl)pentane-1,5-diol Bis(2-hydroxyethyl)aMinotris(hydroxyMethyl)Methane Bis(2-hydroxyethyl)iMinotris(hydroxyMethyl)Methan BIS-HYDROXYETHYL TROMETHAMINE 2,2-Bis(hydroxyMethyl)-2,2',2''-nitrilotriethanol (BIS-TRIS) BIS-TRIS >99% BIS-TRIS Vetec(TM) reagent grade, >=98%, RNase and DNase free COLOREX CLOSTRIDIUM DIFFICILE 90MM INDICATOR PAPER ROTA PH 1-10 ROLL ROSE BENGAL CHLOR.PENASE 90MM DOUBLE-WR. Bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane [Good's buffer component for biological research] 2,2-Bis(hydroxymethyl)-2,2μ,2-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol, 99+%, for biochemistry 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol, for biochemistry 2,2'-[2-Hydroxy-1,1-bis(hydroxymethyl)ethylimino]bisethanol 2,2-Bis(hydroxymethyl)-(2,2',2''-nitrilotrisethanol) 2,2-BIS(HY 2-[Bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)-