PHOSPHOLIPASE A1 FROM ASPERGILLUS ORYZAE

Uses

Phospholipase A1 from Aspergillus oryzae has been used:

• in the preparation of sn-1 and sn-2 C18:1- lysophosphatidylcholine (LPC) regioisomer standards
• as a catalyst for the synthesis 6-O-glucosyl-poly(3-hydroxyalkanoates) in a micro-aqueous system
• to catalyze the synthesis of methyl butanoate and methyl benzoate flavor esters in continuous flow microreactor
• to hydrolyze 17:0 phosphocholine (PC)

General Description

Phospholipase A1 (PLA1) catalyzes the hydrolysis of acyl group from position 1 of lecithin to yield lysolecithin. It is expressed in a wide range of organisms such as rat platelets, bovine brain and testis, hornet venom, bonito muscle and fungi. Gene coding for PLA1 consists of four exons and three short introns spanning 1,056bp of genomic DNA. Mature protein contains 269 aminoacids and two possible N-glycosylation sites (Asn27 and Asn55).