LENTI CRISPR HUMAN HPRT1 POSITIVE CONTROL PLASMID DNA (LV04 VECTOR)
- CAS No.
- Chemical Name:
- LENTI CRISPR HUMAN HPRT1 POSITIVE CONTROL PLASMID DNA (LV04 VECTOR)
- Synonyms
- LENTI CRISPR HUMAN HPRT1 POSITIVE CONTROL PLASMID DNA (LV04 VECTOR)
- CBNumber:
- CB44742027
- Molecular Formula:
- Molecular Weight:
- 0
- MDL Number:
- MOL File:
- Mol file
LENTI CRISPR HUMAN HPRT1 POSITIVE CONTROL PLASMID DNA (LV04 VECTOR) price More Price(1)
LENTI CRISPR HUMAN HPRT1 POSITIVE CONTROL PLASMID DNA (LV04 VECTOR) Chemical Properties,Uses,Production
Uses
Functional Genomics/Screening/Target Validation
General Description
The Lenti CRISPR Human HPRT1 Positive Control Plasmid DNA (LV04 vector) is a critical positive control to monitor transfection efficiency for lentiCRISPRs. The protein encoded by this gene is a transferase which plays a central role in the generation of purine nucleotides. Cells with intact HPRT1 will die upon exposure to 6-TG (Product No. A4882), an antimetabolite used in the treatment of leukemias. Conversely, cells with HPRT1 knocked out will increase over time with 6-TG selection. This targets a validated HPRT1 CRISPR site, which serves as an experimental control for lenti CRISPRs. The lenti CRISPR positive control is a dual-component system consisting of a U6-driven guide RNA plasmid targeting the human HPRT1 gene and hPGK-driven Puromycin and BFP expression. This control requires Cas9 be delivered by a separate vector.
LENTI CRISPR HUMAN HPRT1 POSITIVE CONTROL PLASMID DNA (LV04 VECTOR) Preparation Products And Raw materials
Raw materials
Preparation Products
LENTI CRISPR HUMAN HPRT1 POSITIVE CONTROL PLASMID DNA (LV04 VECTOR) Suppliers
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Merck KGaA | 21-20338288 | ordercn@merckgroup.com | China | 292394 | 58 |
| Supplier | Advantage |
|---|---|
| Merck KGaA | 58 |