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REVERSE TRANSCRIPTASE

REVERSE TRANSCRIPTASE structure

CAS No.: 9068-38-6

Chemical Name:: REVERSE TRANSCRIPTASE

Synonyms: HIV-1 RT;abs60073;revertase;REVERSCRIPT 1;REVERSE TRANSCRIPTASE;HIV REVERSE TRANSCRIPTASE;AMV REVERSE TRANSCRIPTASE;REVERSE TRANSCRIPTASE AMV;RTL reverse transcriptase;M-MLV REVERSE TRANSCRIPTASE

CBNumber:: CB4702207

Molecular Formula:

Molecular Weight:: 0

MDL Number:: MFCD00283754

MOL File:: Mol file

Last updated:2023-04-23 13:52:06

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REVERSE TRANSCRIPTASE Properties

storage temp.	-20°C
form	Liquid
color	colorless

SAFETY

Risk and Safety Statements

WGK Germany	3
HS Code	3504009000

REVERSE TRANSCRIPTASE price More Price(4)

Manufacturer	Product number	Product description	CAS number	Packaging	Price	Updated	Buy
Sigma-Aldrich	M1302	M-MLV Reverse Transcriptase Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis	9068-38-6	40000units	$397	2024-03-01	Buy
Alfa Aesar	J60167	Reverse Transcriptase, murine, Moloney Murine Leukemia Virus	9068-38-6	10kilounit	$123	2021-12-16	Buy
Usbiological	R1992-08	Reverse Transcriptase	9068-38-6	100ug	$460	2021-12-16	Buy
American Custom Chemicals Corporation	ENZ0000287	M-MLV REVERSE TRANSCRIPTASE 95.00%	9068-38-6	5MG	$499.05	2021-12-16	Buy

Product number	Packaging	Price	Buy
M1302	40000units	$397	Buy
J60167	10kilounit	$123	Buy
R1992-08	100ug	$460	Buy
ENZ0000287	5MG	$499.05	Buy

REVERSE TRANSCRIPTASE Chemical Properties,Uses,Production

Description

Enzyme possessed by retroviruses that allows transcription of DNA from an RNA template. Such an enzyme has never been isolated from other sources, but speculation about its possible occurrence continues. Such an enzyme is widely used experimentally in genetic engineering to allow the formation of complementary DNA (cDNA) from purified RNA. The discovery of this enzyme faulted the widely held central dogma, which stated that the information flow in nature was always from DNA to RNA.

Uses

Synthesizes a complementary DNA strand using single-stranded RNA or DNA in the presence of a primer

Uses

Reverse Transcriptase AMV is suitable for:

First- and second-strand cDNA synthesis and synthesis of first strand cDNA for use in subsequent amplification reactions (RT-PCR)
Dideoxy DNA sequencing
Primer extension
RNA sequencing
3′-end labeling of DNA fragments
Generation of single-stranded probes for genomic footprint experiments

Uses

M-MLV Reverse Transcriptase has been used:

for the preparation of cDNA libraries or for first strand cDNA synthesis
for use in a 2-step RT-PCR assay
for the synthesis of cDNA that is further used in cloning

General Description

M-MLV (Moloney murine leukemia virus) reverse transcriptase enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. MoMLV RT is made up of 671 amino acid residues. It is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand.

Purification Methods

This enzyme produces the complementary DNA from the RNA (as template). These are purified by solubilising the virus with non-ionic detergents. Lysed virions are adsorbed on DEAE-cellulose or DEAE-Sephadex columns, and the enzymes are eluted with a salt gradient, then chromatographed on a phosphocellulose column and fractions with enzyme activity are eluted in a salt gradient. They are also purified from other viral proteins by affinity chromatography on a pyran-Sepharose column. [Verna Biochim Biophys Acta 473 1 1977, Smith Methods Enzymol 65 560 1980; see commercial catalogues for other transcriptases.]

REVERSE TRANSCRIPTASE Preparation Products And Raw materials

Raw materials

Preparation Products

REVERSE TRANSCRIPTASE Suppliers

Global( 133)Suppliers

Supplier	Tel	Email	Country	ProdList	Advantage
Shanghai Daken Advanced Materials Co.,Ltd	+86-371-66670886	info@dakenam.com	China	18774	58
career henan chemical co	+86-0371-86658258 +8613203830695	factory@coreychem.com	China	29811	58
Creative Enzymes	1-516-855-7709	info@creative-enzymes.com	United States	8748	58
Hebei Fengjia New Material Co., Ltd	+86-0311-87836622 +86-17333973358	sales06@hbduling.cn	China	8053	58
Nanjing Duly Biotech Co.,Ltd	+undefined18013301590	melody@njduly.com	China	2450	58
Watson Biotechnology Co.,Ltd	+86-18186686046 +86-18186686046	sales01@watsonbiotech.cn	China	5821	58
J & K SCIENTIFIC LTD.	010-82848833 400-666-7788	jkinfo@jkchemical.com	China	96815	76
Sichuan Kulinan Technology Co., Ltd	400-1166-196 18981987031	cdhxsj@163.com	China	11707	57
Chengdu Ai Keda Chemical Technology Co., Ltd.	4008-755-333 18080918076	800078821@qq.com	China	9718	55
Shanghai Aladdin Bio-Chem Technology Co.,LTD	400-400-6206333 18521732826	market@aladdin-e.com	China	25003	65

Supplier	Advantage
Shanghai Daken Advanced Materials Co.,Ltd	58
career henan chemical co	58
Creative Enzymes	58
Hebei Fengjia New Material Co., Ltd	58
Nanjing Duly Biotech Co.,Ltd	58
Watson Biotechnology Co.,Ltd	58
J & K SCIENTIFIC LTD.	76
Sichuan Kulinan Technology Co., Ltd	57
Chengdu Ai Keda Chemical Technology Co., Ltd.	55
Shanghai Aladdin Bio-Chem Technology Co.,LTD	65

View Lastest Price from REVERSE TRANSCRIPTASE manufacturers

Image	Update time	Product	Price	Min. Order	Purity	Supply Ability	Manufacturer
	2020-01-10	REVERSE TRANSCRIPTASE, M-MLV, RNASEH+ 9068-38-6	US $1.00 / KG	1KG	99%	200kg	Career Henan Chemical Co

REVERSE TRANSCRIPTASE, M-MLV, RNASEH+
9068-38-6
US $1.00 / KG
99%
Career Henan Chemical Co

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M-MLV REVERSE TRANSCRIPTASE MOLONEY MURINE LEUKEMIA VIRUS REVERSE TRANSCRIPTASE Nucleotidyltransferase, deoxyribonucleate, RNA-dependent REVERSE TRANSCRIPTASE, FOR MOL. BIOLOGY, DRY ICE, 500 U* REVERSE TRANSCRIPTASE FROM AVIAN*MYELOBL ASTOSIS VIR CYSCRIBE REVERSE TRANSCRIPTASE AMV REVERSE TRANSCRIPTASE, REC., INDUSTRIAL GMP GRADE amv-reverse transcriptase from avian myeloblastosis virus revertase Reverse Trancscriptase, Molony Mouse Leukemia Virus, Recombinant AVIAN MYELOBLASTOSIS VIRUS REVERSE TRANSCRIPTASE HIV REVERSE TRANSCRIPTASE HIV REVERSE TRANSCRIPTASE, RECOMBINANT, E COLI HIV-1 REVERSE TRANSCRIPTASE HIV-1 RT AMV REVERSE TRANSCRIPTASE AMV REVERSE TRANSCRIPTASE (HC) RNA-DIRECTED DNA POLYMERASE REVERSCRIPT 1 REVERSE TRANSCRIPTASE REVERSE TRANSCRIPTASE AMV REVERSE TRANSCRIPTASE, AMV, (HC) REVERSE TRANSCRIPTASE, M-MLV, RNASEH+ M-MLV REVERSE TRANSCRIPTASE, RNASE H MINUS M-MLV REVERSE TRANSCRIPTASE, RNASE H MINUS, POINT MUTANT Revertase, RNA-directed DNA polymerase Reverse Transcriptase from HIV, Recombinant Native Avian myeloblastosis virus AMV-Reverse Transcriptase M-MLV Reverse Transcriptase Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis ToloScript Reverse Transcriptase abs60073 M-MLV Reverse transcriptase BR RTL reverse transcriptase 9068-38-6 Nucleic Acid Amplification Molecular Biology PCR/Amplification Plant Biotechnology Plant Molecular Biology RT-PCR BioChemical Enzyme 2.7.x.x Phosphorus containing groupsMore...Close... AmplificationMolecular Biology cDNA LabelingApplication Index PCRPlant Molecular Biology RT-PCREnzyme Class Index 2.x.x.x Transferases Functional Profiling of Stem Cells Gene Expression&Analysis Molecular Biology Tested Nucleic Acid Amplification PCR/Amplification RT-PCRMolecular Biology Stem Cell Biology