SP6 RNA POLYMERASE

CAS No.
Chemical Name:
SP6 RNA POLYMERASE
Synonyms
SP6 RNA;SP6 RNA POLYMERASE
CBNumber:
CB4778598
Molecular Formula:
Molecular Weight:
0
MDL Number:
MFCD00212692
MOL File:
Mol file
Last updated:2023-04-23 13:52:06

SP6 RNA POLYMERASE Properties

storage temp. -20°C
form solution

SP6 RNA POLYMERASE price More Price(2)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich RPOLSP6-RO SP6 RNA Polymerase from Escherichia coli BL 21/pSR3 1000units $187 2024-03-01 Buy
Sigma-Aldrich RPOLSP6-RO SP6 RNA Polymerase from Escherichia coli BL 21/pSR3 5000units $715 2024-03-01 Buy
Product number Packaging Price Buy
RPOLSP6-RO 1000units $187 Buy
RPOLSP6-RO 5000units $715 Buy

SP6 RNA POLYMERASE Chemical Properties,Uses,Production

Uses

SP6 RNA Polymerase can transcribe RNA from cloned DNA templates that are downstream from an SP6 promoter. The synthesis can be performed with labeled NTPs to generate highly labeled RNA. Synthesized RNA can be used in many applications, including:

  • RNA or DNA blotting techniques
  • In situ hybridization
  • RNase protection studies: Transcripts synthesized by the enzyme are used as precursor RNA for studies on RNA splicing and processing.
  • Synthesis of capped RNA in vitro with addition of m7GpppG or m7GpppA in excess over GTP or ATP during the transcription reaction. The generated antisense RNA can be introduced into cells to suppress the expression of the corresponding genes.
  • The synthesis of antisense RNA probes

General Description

With this system, homogeneously labeled single-stranded RNA can be generated. Transcripts can be non-radioactively labeled with biotin or DIG-11-UTP or radioactively labeled to high specific activity with [α-32P] or [α-35S] labeled nucleotides.

Contents:

  • SP6 RNA Polymerase, ≥20 U/μl, in buffer, pH 7.9
  • Transcription Buffer, 10x concentrated

SP6 RNA POLYMERASE Preparation Products And Raw materials

Raw materials

Preparation Products

SP6 RNA POLYMERASE Suppliers

Global( 78)Suppliers
Supplier Tel Email Country ProdList Advantage
Sigma-Aldrich 021-61415566 800-8193336 orderCN@merckgroup.com China 51471 80
Guangzhou LES biological Technology Co.,Ltd. 13672434928 3307355104@qq.com China 3650 58
Zhejiang Lianshuo Biotechnology Co., Ltd. 18616526224 lianshuo@vip.126.com China 9610 58
Shanghai jingkang bioengineering co., ltd. 021-54721350 13524668266 2881505714@qq.com China 7878 58
Shanghai Kanglang Biotechnology Co., Ltd. 021-61998208 18217752821 sales@klbio.cn China 9617 58
Shanghai Sig Biotechnology Co., LTD 021-57810052 18930344717 2089316240@qq.com China 9396 58
Aleshan (Guangzhou) Biotechnology Co., Ltd 18636736628 Araratbio@163.com China 3342 58
Nantong Feiyu Biological Technology CO.,LTD 0513-68819626; 13813788405 feiyubio2@163.com China 4821 58
Shanghai Yingxin Laboratory Equipment Co., Ltd. 021-021-59178156 17002132182 3146474380@qq.com China 11609 58
Shanghai Guchen Biotechnology Co., LTD 021-34675735 19147740836 1986399151@qq.com China 9849 58
SP6 RNA POLYMERASE SP6 RNA