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4-Chloro-7-nitrobenzofurazan

4-Chloro-7-nitrobenzofurazan Structure
4-Chloro-7-nitrobenzofurazan structure
CAS No.
10199-89-0
Chemical Name:
4-Chloro-7-nitrobenzofurazan
Synonyms
NBD-CL;4-chloro-7-nitrobenzo[c][1,2,5]oxadiazole;4-Chloro-7-nitro-1,2,3-benzoxadiazole;4-CHLORO-7-NITROBENZOFURAZAN;NBD CHLORIDE;4-chloro-7-nitrobenzo-2,1,3-oxadiazole;7-CHLORO-4-NITROBENZO-2-OXA-1,3-DIAZOLE;4-CHLORO-7-NITROBENZOFURAZAN, FOR FLUORE SCENCE;NBC-1;nbd-c1
CBNumber:
CB5173637
Molecular Formula:
C6H2ClN3O3
Molecular Weight:
199.55
MDL Number:
MFCD00005808
MOL File:
10199-89-0.mol
MSDS File:
SDS
Last updated:2024-05-31 19:03:53
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4-Chloro-7-nitrobenzofurazan Properties

Melting point 97-99 °C(lit.)
Boiling point 333.1±45.0 °C(Predicted)
Density 2.0589 (rough estimate)
refractive index 1.5810 (estimate)
storage temp. Inert atmosphere,Room Temperature
solubility Soluble in methanol, dimethylsulfoxide, dimethylformamide and chloroform.
form Crystalline Powder
pka -5.65±0.50(Predicted)
color Yellow to light brown
Water Solubility soluble
BRN 614212
CAS DataBase Reference 10199-89-0(CAS DataBase Reference)
EWG's Food Scores 1
FDA UNII EQF2794IRE
EPA Substance Registry System 2,1,3-Benzoxadiazole, 4-chloro-7-nitro- (10199-89-0)

SAFETY

Risk and Safety Statements

Symbol(GHS)  GHS hazard pictograms
GHS07
Signal word  Warning
Hazard statements  H315-H319
Precautionary statements  P302+P352-P305+P351+P338
Hazard Codes  Xn,Xi
Risk Statements  22-36/38
Safety Statements  36/37/39
RIDADR  UN 2811 6.1/PG 3
WGK Germany  3
RTECS  DF8002400
F  10-21
TSCA  Yes
HS Code  29349990
NFPA 704
1
2 0

4-Chloro-7-nitrobenzofurazan price More Price(42)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 163260 4-Chloro-7-nitrobenzofurazan 98% 10199-89-0 1g $51 2024-03-01 Buy
Sigma-Aldrich 163260 4-Chloro-7-nitrobenzofurazan 98% 10199-89-0 5g $71.3 2024-03-01 Buy
TCI Chemical A5592 NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole)[for HPLC Labeling] >98.0%(T) 10199-89-0 1g $42 2024-03-01 Buy
TCI Chemical A5592 NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole)[for HPLC Labeling] >98.0%(T) 10199-89-0 5g $121 2024-03-01 Buy
Alfa Aesar A14165 4-Chloro-7-nitrobenzofurazan, 99% 10199-89-0 5g $65.2 2024-03-01 Buy
Product number Packaging Price Buy
163260 1g $51 Buy
163260 5g $71.3 Buy
A5592 1g $42 Buy
A5592 5g $121 Buy
A14165 5g $65.2 Buy

4-Chloro-7-nitrobenzofurazan Chemical Properties,Uses,Production

Chemical Properties

yellow to light brown crystalline powder

Uses

4-Chloro-7-nitrobenzofurazan is used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines. It is utilized in the preparation of fluorescent phospholipid-derivative, hydroxynaphthofurazan and 4-chloro-7-nitrobenzofurazan- didecanoylphosphatidylethanolamine. It serves as a fluorescent reagent to label free sulfhydryls and N-terminals within proteins and trapping agent for cysteine sulfenic acid. It is a sensitive chromogenic and fluorogenic reagent. It reacts with trans-1-methoxy-3-(trimethylsilyloxy)-1,3-butadiene (Danishefsky's diene) to prepare regioselectively the silyl enol ether. Further, it is used to label peptides, proteins and other biomolecules. In addition it is used in the synthesis of fluorescent phospholipid-derivative, NBD-didecanoylphosphatidylethanolamine, functionalized hydroxynaphthofurazan and 7-nitrobenzofurazan (NBD)-labeled maleimide.

Uses

4-Chloro-7-nitrobenzofurazan is nonfluorescent until it reacts with primary or secondary amines to produce a fluorescent product. 4-Chloro-7-nitrobenzofurazan has been extensively used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines.

Definition

ChEBI: A benzoxadiazole that is 2,1,3-benzoxadiazole which is substituted at position 4 by chlorine and at position 7 by a nitro group.

General Description

4-Chloro-7-nitrobenzofurazan (NBD-Cl) is a highly sensitive chromogenic and fluorogenic reagent. It is reported to react spontaneously with trans-1-methoxy-3-(trimethylsilyloxy)-1,3-butadiene (Danishefsky′s diene), to afford regioselectively the silyl enol ether, via normal electron-demand Diels-Alder (NEDDA) reaction. A study of the molecular structure, vibrational spectra and NBO (Natural Bond Orbital) analysis of NBD–Cl has been undertaken. Its utility as a pre-column derivatization agent has been examined.

in vitro

4-Chloro-7-nitrobenzofurazan(NBD chloride) forms highly fluorescent derivatives for detection of all the protein amino acids. In addition, NBD-Cl provides a simple and sensitive method for determination of N-terminal amino acids. The differences in intensity and color of fluorescence can be used to advantage to identify prolyl peptides.

Purification Methods

Wash the solid with H2O, and it recrystallises from aqueous EtOH (1:1) as pale yellow needles. It sublimes in a vacuum [Gosh & Whitehouse Biochem J 108 155 1968, UV, NMR: Bolton et al. J Chem Soc 1004 1966].

4-Chloro-7-nitrobenzofurazan synthesis

7116-16-7
10199-89-0
Synthesis of 4-Chloro-7-nitrobenzofurazan from 4-CHLOROBENZOFURAZAN

4-Chloro-7-nitrobenzofurazan Preparation Products And Raw materials

Raw materials

Preparation Products

4-Nitro-7-piperidin-1-yl-2,1,3-benzoxadiazole 2-[(7-Nitro-2,1,3-benzoxadiazol-4-yl)thio]ethanaMine

4-Chloro-7-nitrobenzofurazan Suppliers

Global( 241)Suppliers
Supplier Tel Email Country ProdList Advantage
Hebei Mojin Biotechnology Co., Ltd 		+86 13288715578 +8613288715578 sales@hbmojin.com China 12840 58
Henan Tianfu Chemical Co.,Ltd. 		+86-0371-55170693 +86-19937530512 info@tianfuchem.com China 21634 55
ATK CHEMICAL COMPANY LIMITED 		+undefined-21-51877795 ivan@atkchemical.com China 32957 60
Hefei TNJ Chemical Industry Co.,Ltd. 		+86-0551-65418679 +8618949832763 info@tnjchem.com China 2986 55
career henan chemical co 		+86-0371-86658258 +8613203830695 sales@coreychem.com China 29884 58
Hebei Weibang Biotechnology Co., Ltd 		+8615531157085 abby@weibangbio.com China 8812 58
Chongqing Chemdad Co., Ltd 		+86-023-6139-8061 +86-86-13650506873 sales@chemdad.com China 39894 58
Alchem Pharmtech,Inc. 		8485655694 sales@alchempharmtech.com United States 63687 58
CONIER CHEM AND PHARMA LIMITED 		+8618523575427 sales@conier.com China 49374 58
Fuxin Pharmaceutical 		+86-021-021-50872116 +8613122107989 contact@fuxinpharm.com China 10032 58
Supplier Advantage
Hebei Mojin Biotechnology Co., Ltd 		58
Henan Tianfu Chemical Co.,Ltd. 		55
ATK CHEMICAL COMPANY LIMITED 		60
Hefei TNJ Chemical Industry Co.,Ltd. 		55
career henan chemical co 		58
Hebei Weibang Biotechnology Co., Ltd 		58
Chongqing Chemdad Co., Ltd 		58
Alchem Pharmtech,Inc. 		58
CONIER CHEM AND PHARMA LIMITED 		58
Fuxin Pharmaceutical 		58

View Lastest Price from 4-Chloro-7-nitrobenzofurazan manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
4-Chloro-7-nitro-1,2,3-benzoxadiazole pictures 2024-05-31 4-Chloro-7-nitro-1,2,3-benzoxadiazole
10199-89-0
 US $1150.00 / g 1g 98 500 Kg R&D Scientific Inc.
4-Chloro-7-nitrobenzofurazan pictures 2023-09-06 4-Chloro-7-nitrobenzofurazan
10199-89-0
 US $0.00 / KG 1KG 99% 50000KG/month Hebei Mojin Biotechnology Co., Ltd
4-Chloro-7-nitrobenzofurazan pictures 2023-01-11 4-Chloro-7-nitrobenzofurazan
10199-89-0
 US $10.00 / KG 1KG 99% 10 mt Hebei Weibang Biotechnology Co., Ltd

4-Chloro-7-nitrobenzofurazan Spectrum

4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole(10199-89-0)MS4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole(10199-89-0)1HNMR4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole(10199-89-0)IR14-Chloro-7-nitrobenzo-2-oxa-1,3-diazole(10199-89-0)IR24-Chloro-7-nitrobenzo-2-oxa-1,3-diazole(10199-89-0)Raman

10199-89-0(4-Chloro-7-nitrobenzofurazan)Related Search:

4-Chloro-7-nitrobenzofurazan Nitrobenzene BENZOFURAZAN N-BENZYL-4-NITRO-2,1,3-BENZOXADIAZOL-5-AMINE 7-CHLORO-4-NITRO-5-PYRROLIDIN-1-YL-BENZO[1,2,5]OXADIAZOLE
7-CHLORO-4-NITRO-5-PIPERIDINO-2,1,3-BENZOXADIAZOLE 5-AMINO-7-CHLORO-4-NITROBENZOFURAZAN 7-CHLORO-N,N-DIETHYL-4-NITRO-2,1,3-BENZOXADIAZOL-5-AMINE 2-(7-CHLORO-4-NITRO-BENZO[1,2,5]OXADIAZOL-5-YLAMINO)-ETHANOL 3-(7-CHLORO-4-NITRO-BENZO[1,2,5]OXADIAZOL-5-YLAMINO)-PROPAN-1-OL
AKOS BB-7022 7-CHLORO-5-(4-METHYL-PIPERAZIN-1-YL)-4-NITRO-BENZO[1,2,5]OXADIAZOLE 7-CHLORO-5-MORPHOLIN-4-YL-4-NITRO-BENZO[1,2,5]OXADIAZOLE (7-CHLORO-4-NITRO-BENZO[1,2,5]OXADIAZOL-5-YL)-PYRIDIN-2-YL-AMINE N-[4-(7-CHLORO-4-NITRO-BENZO[1,2,5]OXADIAZOL-5-YLAMINO)-PHENYL]-ACETAMIDE
TIMTEC-BB SBB005650 (7-CHLORO-4-NITRO-BENZO[1,2,5]OXADIAZOL-5-YL)-DIMETHYL-AMINE 4-CHLORO-5,7-DINITROBENZOFURAZAN

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7-CHLORO-4-NITROBENZ-2-OXA-1-3-DIAZOLECR YSTALLINE 4-Chloro-7-nitrobenzo-2,1,3-oxadiazole~NBDchloride 2,1,3-Benzoxadiazole, 4-chloro-7-nitro- NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling] 4-chloro-7-nitrobenzofurazane 4-Chloro-7-nitro-1,2,3-benzoxadiazole, NBD-chloride NBC-1 NBD chloride,98% NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole) 4-Chloro-7-nitro-1,2 7-Chloro-4-nitro-2,1,3-benzoxadiazole NSC 228140 4-Chloro-7-nitrobenzofurazan 4-Chloro-7-nitro-2,1,3-benzoxadiazole 4-chloro-7-nitro-benzofuraza 4-nitro-7-chlorobenzofurazan nbd-c1 7-CHLORO-4-NITRO-2,1,3-BENZOXADIAZOL 7-CHLORO-4-NITROBENZOFURAZAN AKOS BC-0686 4-CHLORO-7-NITRO-2,1,3-BENZOXADIAZOLE 4-CHLORO-7-NITROBENZ-2-OXA-1,3-DIAZOLE 4-CHLORO-7-NITRO-BENZO[1,2,5]OXADIAZOLE 4-CHLORO-7-NITROBENZO-2-OXA-1,3-DIAZOLE 4-CHLORO-7-NITROBENZUFURAZAN 4-Chloro-7-nitro-1,2,3-benzoxadiazole (NBD-Cl ) 4-Chloro-7-nitrobenzo-2-furazan, NBF-Cl 1-Chloro-4-nitrobenzoxadiazole LABOTEST-BB LT00238516 TIMTEC-BB SBB005345 7-CHLORO-4-NITROBENZ-2-OXA-1,3-DIAZOLE Benzofurazan, 4-chloro-7-nitro- Shokat-update-comp17 4-Chloro-7-nitro-2,1,3-benzoxadiazole 99% NBD-Cl [4-Chloro-7-nitrobenzofurazan] *CAS 10199-89-0* NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole)[for HPLC Labeling]> NBD-Cl [4-Chloro-7-nitrobenzofurazan oro-7-nitrobenzo-2-oxa-1,3-diazoL 4-CHLORO-7-NITROBENZOFURAZAN, FOR FLUORE SCENCE 4-chloro-7-nitrobenzo-2,1,3-oxadiazole 4-chloro-7-nitrobenzo[c][1,2,5]oxadiazole 7-CHLORO-4-NITROBENZO-2-OXA-1,3-DIAZOLE 4-CHLORO-7-NITROBENZOFURAZAN NBD CHLORIDE NBD-CL 4-Chloro-7-nitro-1,2,3-benzoxadiazole 4-Chloro-7-nitro-2,1,3-benzoxadiazole(NBD-Cl) 4-Choro-7-nitrobenzofurazan 4-Chloro-7-nitrobenzo-2-furazan 4-Chloro-7-nitrobenzo-2-oxa-1,3-oxadiazole 10199-89-0 C6H2ClN3O3 Mercapto Group Labeling Reagents for Fluorescence HPLC HPLC Labeling Reagents Fluorescence Detection (HPLC Labeling Reagents) Amino Group Labeling Reagents for Fluorescence HPLC Functional Group Reactive Labels Functional Group Reactive Labels for Immunoassays Fluorescent Probes, Labels, Particles and Stains