Bleomycin
- CAS No.
- 11056-06-7
- Chemical Name:
- Bleomycin
- Synonyms
- BLM;BLEO;BLEXANE;Bleocin;Bleomycin;BLENOXANE;bleomicin;Bleocinbase;DNA helicase;Bleo Bleocin
- CBNumber:
- CB5412174
- Molecular Formula:
- C110H168N34O46S7
- Molecular Weight:
- 2927.17
- MDL Number:
- MFCD00070310
- MOL File:
- 11056-06-7.mol
- MSDS File:
- SDS
| storage temp. | 2-8°C |
|---|---|
| solubility | H2O: 20 mg/mL |
| form | powder |
| color | white |
| EWG's Food Scores | 2 |
| FDA UNII | 40S1VHN69B |
| ATC code | L01DC01 |
| IARC | 2B (Vol. 26, Sup 7) 1987, 1 (Vol. 76, 100A) 2012 |
| EPA Substance Registry System | Bleomycin (11056-06-7) |
SAFETY
Risk and Safety Statements
| Hazard Codes | T |
|---|---|
| Risk Statements | 46-40 |
| Safety Statements | 53-36/37-45 |
| WGK Germany | 3 |
| RTECS | EC5991990 |
| F | 10 |
| Toxicity | dog,LD,oral,> 100mg/kg (100mg/kg),Japanese Journal of Antibiotics. Vol. 28, Pg. 1, 1975. |
Bleomycin price
Bleomycin Chemical Properties,Uses,Production
Description
Bleomycin is a complex of no less than 16 glycopeptide antibiotics made from the family Streptomyces verticilus, which have different R groups. Bleomycines exhibit antitumor, antiviral, and antibacterial activity. When bound to DNA, they disturb the spiraling of both single and double strands of DNA. To a lesser degree, they inhibit RNA and protein synthesis. It is administered both intravenously and intramuscularly.
Originator
Bleomycin Hydrochloride,Nippon Kayaku, Co.,Japan
Uses
Bleomycin sulfate USP (Blenoxane)is used to traet squamous cell carcinoma of head, neck, esophagus, skin, GU tract; testicular tumor; Hodgkin’s lymphomas.
Uses
It is used for lymphomas, carcinomas, and sarcomas.
Uses
Antineoplastic for solid tumors.
Definition
A species of bleomycin noted for its adverse pulmonary effects in humans. It is a complex of related glycopeptide antibiotics from Streptomyces verticillus consisting of bleomycin A2 and B2.
Indications
The bleomycins are a group of glycopeptides that are isolated from Streptomyces verticillus. The clinical preparation, bleomycin sulfate (Blenoxane), is a mixture of several components. Bleomycin binds to DNA, in part through an intercalation mechanism, without markedly altering the secondary structure of the nucleic acid. The drug produces both single- and double-strand scission and fragmentation of DNA. It is thought that the bleomycins, which are avid metal-chelating agents, form a bleomycin–Fe ++ complex that can donate electrons to molecular oxygen, thus forming the superoxide and hydroxyl free radicals. It is these highly reactive intermediates that attack DNA and produce DNA strand breakage and maximum cytotoxicity in the late G2 and early M-phases of the cell cycle.
Manufacturing Process
To a medium having a composition of 6.4 % of millet jelly, 0.5 % of glucose,
3.5 % of soybean powder, 0.75 % of corn steep liquor, 0.3 % of sodium
chloride, 0.1 % of potassium secondary phosphate, 0.05 % of zinc sulfate,
0.01 % of copper sulfate, 0.2 % of sodium nitrate and 0.01 % of Toho No. 1
(trade name for a surface active agent composed of polyoxyethylene
manufactured by Toho Chemical Industry Co. Ltd., Japan) was added 3-aminopropyl-
dimethylsulfonium bromide hydrobromate in a proportion of 0.4 mg/ml
to adjust the pH of the medium to 6.5.
Each 100 ml of the thus treated medium was separately charged into a
Sakaguchi flask and was then sterilized. Subsequently, Streptomyces
verticillus (ATCC No. 15003) was inoculated in the medium and was cultured
at 27°C for 8 days with stirring at 130 r.p.m. Thereafter, the culture liquors
(4.5 L) were collected and filtered to obtain 3.0 L of a filtrate (potency 38.8
mg/ml, total potency 416.4 mg). This culture filtrate was passed through and
adsorbed on a column packed with 200 ml of Amberlite IRC-50 and was
washed with water and was eluted with 0.5 N hydrochloric acid. 1.0 L of the
eluate was neutralized, was passed through and adsorbed on a column packed
with 100 ml of active carbon, was washed and was then eluted by use of a
1:1 (by volume) mixture of acetone - 0.02 N aqueous hydrochloric acid
solution, and fractions active to Mycobacterium 607 were collected and
concentrated to dryness. The resulting residue was dissolved in 5 ml of an 80
% aqueous methanol solution and was charged into a column packed with 30
ml of neutral alumina, followed by elution with an 80 % aqueous methanol
solution. Subsequently, bleomycin-containing fractions were collected and
concentrated to dryness to obtain 195 mg of bleomycin hydrochloride
(potency 650.7 mcg/mg, total potency 172 mg). The yield from the culture
filtrate was 30.5 %.
brand name
Blenoxane (Bristol-Myers Squibb).
Therapeutic Function
Antibiotic
General Description
Bleomycin is a glycopeptide antibiotic complex isolatedfrom Streptomyces verticillus initially by Umezawa.Atleast 13 different fractions of bleomycin have been isolatedwith the clinically used product (Blenoxane) being a mixtureof predominantly A22 (55%–70%) and B2 (25%–32%)fractions.Of these fractions, A2 appears to possessthe greatest antineoplastic activity. Copper is found inthe naturally occurring material, and its removal is importantfor the material used clinically because it significantlyreduces activity.
Bleomycin is notable for its lack of myelotoxicity, andthis allows it to be combined with other myelosuppressantswithout a resulting additive effect. The acute toxicities seenwith bleomycin are erythema (reddening of the skin), hyperpigmentation(skin darkening) found predominately on theextremities, and pulmonary toxicity. The pulmonary toxicitymay first occur as pneumonitis (inflammation of lung tissue),which normally responds to glucocorticosteroid therapy.Chronic pulmonary toxicity is expressed as pulmonaryfibrosis, which is irreversible and limits utility of the agent.
General Description
Colorless or yellowish powder. Possible bluish color depending on copper content.
Air & Water Reactions
Water soluble
Hazard
Possible carcinogen.
Fire Hazard
Flash point data for Bleomycin are not available. Bleomycin is probably nonflammable.
Pharmaceutical Applications
The drug Bleomycin (BLM) is successfully used as an anticancer agent, and is known to cause fragmentation
of the DNA. The drug is used for the treatment of testicular cancer, non-Hodgkin’s lymphoma, Hodgkin’s
lymphoma and cancers of the head and neck area (Cancer research UK). The name Bleomycin describes a
family of water-soluble antibiotics that can be isolated from the bacterium Streptomyces verticillus. All family
members contain the same core structure, a sulfur-containing polypeptide chain, and are only differentiated
by a small side group and the sugar moiety.
BLM was discovered 1966 by Umezawa et al. when they screened the filtrate of S. verticillus for cytotoxic
activity. The therapeutically active forms of BLM are BLM A2
and B2, which differ only in the side chain. BLM is believed to exhibit its anticancer activity by DNA degradation, a process that is dependent on
the presence of molecular oxygen, and the binding of a metal to BLM to form the so-called ‘activated BLM
complex’.
The structure of BLM consists of several biologically important units, each contributing to its anticancer
activity. Two structural units of importance to highlight are the metal-binding site and the DNA-binding
site. It is believed that the intercalation of DNA by BLM occurs via the C-terminus, which contains two
thiazole rings and the positively charged sulfonium salt. The positive charges of the sulfur atom can interact
with the negatively charged phosphate backbones of the DNA. The metal-binding site can be found at the
N-terminus and contains deprotonated amide and histidine groups. The metal is coordinated in a square planar
complex, where a primary amine group occupies the axial position. It can coordinate to a variety of
metals such as Cu2+, Co2+, Zn2+ and Fe2+, but it shows the highest binding affinity to Fe2+. The metal
chelation and subsequent activation of molecular oxygen is crucial to the antiproliferative activity of BLM.
The carbohydrate core seems to be less involved in the direct anticancer activity. Nevertheless, it has been
suggested that it regulates the cellular uptake and indirectly regulates the anticancer activity.
Mechanism of action
Bleomycin is poorly absorbed orally, but it can be given by various parenteral routes. Its plasma half-life is not affected by renal dysfunction as long as creatinine clearance is greater than 35 mL/minute. Bleomycin hydrolase, which inactivates bleomycin, is an enzyme that is abundant in liver and kidney but virtually absent in lungs and skin; the latter two organs are the major targets of bleomycin toxicity. It is thought that bleomycin-induced dermal and pulmonary toxicities are related to the persistence of relatively high local concentrations of active drug.
Clinical Use
Bleomycin, in combination with cisplatin or etoposide, is important as part of the potentially curative combination chemotherapy of advanced testicular carcinomas. Bleomycin is used in some standard regimens for the treatment of Hodgkin’s and non-Hodgkin’s lymphomas, and it is useful against squamous cell carcinomas of the head and neck, cervix, and skin.
Side effects
A potentially fatal lung toxicity occurs in 10 to 20% of patients receiving bleomycin. Patients particularly at risk are those who are over 70 years of age and have had radiation therapy to the chest. Rarely, bleomycin also may cause allergic pneumonitis. Bleomycin skin toxicity is manifested by hyperpigmentation, erythematosus rashes, and thickening of the skin over the dorsum of the hands and at dermal pressure points, such as the elbows. Many patients develop a low-grade transient fever within 24 hours of receiving bleomycin. Less common adverse effects include mucositis, alopecia, headache, nausea, and arteritis of the distal extremities.
Drug interactions
Potentially hazardous interactions with other drugs
Antipsychotics: avoid clozapine, increased risk of
agranulocytosis.
Cytotoxics: increased pulmonary toxicity with
cisplatin and brentuximab, avoid with brentuximab;
in combination with vinca alkaloids can lead to
Raynaud’s syndrome and peripheral ischaemia.
Live vaccines: avoid concomitant use.
Metabolism
The mechanism for bio-transformation is not yet fully known. Inactivation takes place during enzymatic breakdown by bleomycin hydrolase, primarily in plasma, liver and other organs and, to a much lesser degree, in skin and lungs. About 60-70% of the administered drug is excreted unchanged in the urine, probably by glomerular filtration. Approximately 50% is recovered in the urine in the 24 hours following an IV or IM injection. The rate of excretion, therefore, is highly influenced by renal function; concentrations in plasma are greatly elevated if usual doses are given to patients with renal impairment with only up to 20% excreted in 24 hours.
Bleomycin Preparation Products And Raw materials
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Hangzhou ICH Biofarm Co., Ltd | +86-0571-28186870; +undefined8613073685410 | sales@ichemie.com | China | 998 | 58 |
| career henan chemical co | +86-0371-86658258 +8613203830695 | sales@coreychem.com | China | 29886 | 58 |
| Chongqing Chemdad Co., Ltd | +86-023-6139-8061 +86-86-13650506873 | sales@chemdad.com | China | 39894 | 58 |
| CONIER CHEM AND PHARMA LIMITED | +8618523575427 | sales@conier.com | China | 49374 | 58 |
| Creative Enzymes | 1-516-855-7709 | info@creative-enzymes.com | United States | 8748 | 58 |
| Hefei TNJ Chemical Industry Co.,Ltd. | +86-0551-65418671 +8618949823763 | sales@tnjchem.com | China | 34553 | 58 |
| Wuhan Fortuna Chemical Co., Ltd | +86-027-59207850 | info@fortunachem.com | China | 5986 | 58 |
| Baoji Guokang Healthchem co.,ltd | +8615604608665 15604608665 | dominicguo@gk-bio.com | CHINA | 9414 | 58 |
| Alfa Chemistry | Info@alfa-chemistry.com | United States | 24072 | 58 | |
| LEAP CHEM CO., LTD. | +86-852-30606658 | market18@leapchem.com | China | 24727 | 58 |
View Lastest Price from Bleomycin manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2024-11-02 | Bleomycin
11056-06-7
|
US $0.00-0.00 / mg | 1mg | 98% | 10g/month | WUHAN FORTUNA CHEMICAL CO., LTD | |
 |
2023-11-01 | Bleomycin
11056-06-7
|
US $0.00-0.00 / kg | 1kg | 99.9% | 20 tons | Hangzhou ICH Biofarm Co., Ltd | |
 |
2019-07-06 | Bleomycin
11056-06-7
|
US $1.00 / KG | 1KG | 98% | 1kg,5kg,100kg | Career Henan Chemical Co |
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