Biological Activity
FAM65B (family with sequence similarity 65, member B) is recognized as a Ras homolog gene family, member A (RHOA) inhibitor and is involved in polarization. FAM65B regulates RhoA activity with the help of forkhead box O transcription factors. It binds to RhoA and blocks the GTP loading to RhoA. FAM65B deficiency in neutrophils leads to an increase in RhoA activity and causes defects in chemotaxis directionality and adhesion to endothelial cells under flow. It is involved in chemokine-stimulated migration and adhesion of Jurk at cells. Phosphorylation stabilizes FAM65B. FAM65B is a component of the inner ear and is involved in the process of hearing. Mutation in the gene is known to be associated with hearing loss in humans. FAM65B is associated with myotube formation and in the regulation of cell adhesion, polarization, and migration. Overexpression of the protein in myogenic and non-myogenic cells results in the formation of cellular protrusions. Hence, FAM65B might be involved in myoblast migration or cytoskeletal protein rearrangements th at accompany myogenic differentiation.
