Apoptotic DNA-Ladder Kit
- CAS No.
- Chemical Name:
- Apoptotic DNA-Ladder Kit
- Synonyms
- Apoptotic DNA-Ladder Kit
- CBNumber:
- CB64669845
- Molecular Formula:
- Molecular Weight:
- 0
- MDL Number:
- MOL File:
- Mol file
Last updated:2022-08-15 17:36:37
SAFETY
Risk and Safety Statements
Symbol(GHS) | ![]() ![]() GHS05,GHS07 |
---|---|
Signal word | Danger |
Hazard statements | H302-H315-H318-H412 |
Precautionary statements | P264-P270-P273-P280-P305+P351+P338+P310-P501 |
Apoptotic DNA-Ladder Kit Chemical Properties,Uses,Production
Uses
The Apoptotic DNA-Ladder Kit provides rapid isolation of DNA, which can be analyzed and characterized by gel electrophoresis for the determination of apoptotic cell death.
General Description
This kit provides a way to isolate apoptotic DNA fragments for DNA ladder analysis. The purification method used is much faster than other DNA purification methods (e.g., phenol/chloroform extraction, DNA precipitation). Purified DNA can be mixed directly with gel loading buffer and analyzed on an agarose gel.
The study of cell death involves characterizing mortality as apoptotic or necrotic. Apoptosis can be characterized by:
- Prelytic, non-random fragmentation of DNA (“ladder” pattern after agarose-gel electrophoresis)1
- Formation of membrane-bound vesicles (or “apoptotic bodies”)
- Cell shrinkage due to a concentration of cytoplasm
Similarly, necrosis (or physiological cell death) is characterized by
- Random digestion of DNA (DNA smear after agarose-gel electrophoresis)
- Swollen organelles and cells, resulting from loss of membrane integrity and cell lysis
- Postlytic DNA fragmentation
As these differences indicate, the classification of cell death can be accomplished through observing cell morphology, or less subjectively, by analyzing genomic DNA. Resolving the DNA on a gel provides a quick and documentable form of data to differentiate between apoptosis and necrosis.
Apoptotic DNA-Ladder Kit Preparation Products And Raw materials
Raw materials
Preparation Products
Apoptotic DNA-Ladder Kit Suppliers
Global( 18)Suppliers
Supplier | Tel | Country | ProdList | Advantage | |
---|---|---|---|---|---|
Sigma-Aldrich | 021-61415566 800-8193336 | orderCN@merckgroup.com | China | 51471 | 80 |
Supplier | Advantage |
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Sigma-Aldrich | 80 |
Apoptotic DNA-Ladder Kit