Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse
- CAS No.
- Chemical Name:
- Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse
- Synonyms
- Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse
- CBNumber:
- CB82101754
- Molecular Formula:
- Molecular Weight:
- 0
- MDL Number:
- MOL File:
- Mol file
Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse price
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | G6539 | Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse clone GFP-20, ascites fluid | 25μL | $160 | 2023-06-20 | Buy | |
| Sigma-Aldrich | G6539 | Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse clone GFP-20, ascites fluid | 100μL | $480 | 2023-06-20 | Buy | |
| Sigma-Aldrich | G6539 | Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse clone GFP-20, ascites fluid | 0.2ML | $630 | 2023-06-20 | Buy |
Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse Chemical Properties,Uses,Production
Uses
Monoclonal Anti-Green Fluorescent Protein (GFP) recognizes GFP (27 kDa) using immunoblotting, dot blot, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation and enzyme linked immunosorbent assay (ELISA). The antibody reacts with fusion proteins expressed by prokaryotes expression vectors.
General Description
Monoclonal Anti-Green Fluorescent Protein (GFP) (mouse IgG1 isotype) is derived from the GFP-20 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a GFP tagged fusion protein. GFP is a 27 kDa (238 amino acid) protein, derived from the bioluminescent jellyfish Aequorea victoria, in which light is produced when energy is transferred from the Ca2+-activated photoprotein aequorin to GFP. GFP is a unique tool to monitor dynamic processes in a variety of living cells or organisms. When expressed in either eukaryotic or prokaryotic cells and illuminated by blue or UV light, GFP yields a bright green fluorescence. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. Exogenous substrates and cofactors are not required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP signals can be quantified by flow cytometry, confocal scanning laser microscopy, and fluorometric assays. Indeed, many recombinant proteins have been engineered with GFP tags to facilitate the detection, isolation and purification of the proteins.
Biochem/physiol Actions
Green fluorescent protein, produced in the jellyfish, Aequorea victoria, acts as secondary fluorescent protein in an energy transfer reaction to produce green light. It ability to produce a highly visible, internal fluorophore is widely applicable as marker for gene expression and to produce chimeric proteins to understand protein biochemistry.
Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse Preparation Products And Raw materials
Raw materials
Preparation Products
Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse Suppliers
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Sigma-Aldrich | 021-61415566 800-8193336 | orderCN@merckgroup.com | China | 51471 | 80 |
| Supplier | Advantage |
|---|---|
| Sigma-Aldrich | 80 |