vector

vector Properties

storage temp.	-20°C
form	buffered aqueous solution

vector price More Price(110)

Manufacturer	Product number	Product description	Packaging	Price	Updated	Buy
Sigma-Aldrich	PP2380	Bacterial Signal Peptide Vector Set plasmid vectors for molecular cloning	1KIT	$4440	2024-03-01	Buy
Sigma-Aldrich	OGS542	PSF-TEFI-TPI1-NEO/G418 - G418 YEAST SELECTION PLASMID plasmid vector for molecular cloning	5μG	$419	2023-06-20	Buy
Sigma-Aldrich	OGS530	PSF-TEFI-HIS3 - HISTIDINE YEAST SELECTION PLASMID plasmid vector for molecular cloning	5μG	$446	2023-06-20	Buy
Sigma-Aldrich	OGS502	PSF-LACI - LACI PROMOTER BACTERIAL VECTOR plasmid vector for molecular cloning	5μG	$419	2023-06-20	Buy
Sigma-Aldrich	OGS1867	PSF-TEF1-NH2-A-FACTORFL-10HIS - ALPHA FACTOR SECRETION AND 10HIS TAG PLASMID plasmid vector for molecular cloning	5μG	$473	2023-01-07	Buy

Product number	Packaging	Price	Buy
PP2380	1KIT	$4440	Buy
OGS542	5μG	$419	Buy
OGS530	5μG	$446	Buy
OGS502	5μG	$419	Buy
OGS1867	5μG	$473	Buy

vector Chemical Properties,Uses,Production

Description

Plasmids are closed circles of double-stranded DNA, ranging in size from 1 to 200 kilobases, found in many bacteria and in a few eukaryotic cells. They frequently carry genes conferring antibiotic resistance; infective drug resistance, originally discovered in Shigella, is due to plasmids. Plasmids are widely used as carriers of cloned genes, for example the E. coli plasmid pBR322, and numbers of such plasmids may be amplified by treating the donor cells with chloramphenicol, which stops host cell DNA synthesis but not that of the plas_x0002_mid. Some bacteriophages may exist either as integrated or free copies in the cell, and either case can be termed plasmids. Yeast cells have been found to harbour some plasmids, and the Ti plasmid of Agrobacterium can survive in either bacterial or plant cells.

Uses

Cloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.

Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.

The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.

Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.

General Description

Expression in bacterial cells. The RecA promoter is a very strong constitutive promoter. It is approximately 650 x stronger than the constitutive AraBAD promoter that we also sell. The RecA promoter is normally regulated by the repressor LexA. In this promoter this binding site has been ablated to enable constitutive expression. This plasmid is used when the T7 terminator and the SV40 poly-adenylation sites that are found within most of our vectors are not required.

Promoter Expression Level: This plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.

vector Preparation Products And Raw materials

Raw materials

Preparation Products

vector Suppliers

Global( 4)Suppliers

Supplier	Tel	Email	Country	ProdList	Advantage
Shanghai Yubo Biotechnology Co., Ltd.	--	344843571@qq.com	CHINA	6322	58
Beijing Shengke Boyuan Biotechnology Co., Ltd.	--	978560024@qq.com	CHINA	6011	58
Shanghai Lewen Biotechnology Co., Ltd.	--	luwenbio@163.com	CHINA	36	58
Riedel-de Haen AG	--		United States	6825	87

Supplier	Advantage
Shanghai Yubo Biotechnology Co., Ltd.	58
Beijing Shengke Boyuan Biotechnology Co., Ltd.	58
Shanghai Lewen Biotechnology Co., Ltd.	58
Riedel-de Haen AG	87

PSF-TEF1-NH2-T7-THR - N-TERMINAL T7 TAG YEAST PLASMID PSF-TEF1-NH2-6HIS-TEV - N-TERMINAL 6 HIS TAG YEAST PLASMID PSF-TEF1-NH2-FLAG?-TEV - N-TERMINAL FLAG? TAG YEAST PLASMID PSF-TEF1-NH2-FLAG?-3C - N-TERMINAL FLAG? TAG YEAST PLASMID PSF-TEF1-NH2-GST-3C - N-TERMINAL GST TAG YEAST PLASMID PSF-TEF1-NH2-MBP-3C - N-TERMINAL MBP TAG YEAST PLASMID PSF-TEF1-NH2-STREP-6HIS-EKT - N-TERMINAL STREP AND 6 HIS DUAL TAG YEAST PLASMID PSF-CMV-PURO-NH2-INSULINSP-6HIS-TEV - INSULIN SECRETION AND 6 HIS TAG PLASMID PSF-CMV-PURO-NH2-INSULINSP-6HIS-EKT - INSULIN SECRETION AND 6 HIS TAG PLASMID PSF-CMV-COOH-EKT-GST2 - C-TERMINAL GST PLASMID PSF-CMV-PURO-NH2-INSULINSP-CMYC-THR - INSULIN SECRETION AND CMYC TAG PLASMID PSF-CMV-PURO-NH2-6HIS-CAT-3C - N-TERMINAL 6 HIS AND CAT DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-COOH-3C-CAT-6HIS - C-TERMINAL 6 HIS AND CAT DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-COOH-DAGFP - C-TERMINAL DAGFP TAG MAMMALIAN PLASMID PSF-CMV-PURO-COOH-FRCFP - C-TERMINAL CFP TAG MAMMALIAN PLASMID PSF-CMV-PURO-COOH-RLUC - C-TERMINAL RLUC TAG MAMMALIAN PLASMID PSF-CMV-PURO-NH2-6HIS-CAT-TEV - N-TERMINAL 6 HIS AND CAT DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-NH2-6HIS-DAGFP-3C - N-TERMINAL 6 HIS AND DAGFP DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-NH2-6HIS-FRCFP-3C - N-TERMINAL 6 HIS AND CFP DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-NH2-6HIS-BETAGAL-3C - N-TERMINAL 6 HIS AND BETA GAL DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-COOH-THR-FLUC-6HIS - C-TERMINAL 6 HIS AND FLUC DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-NH2-6HIS-CMYC-EKT - N-TERMINAL 6 HIS AND CMYC DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-COOH-EKT-CMYC-6HIS - C-TERMINAL 6 HIS AND CMYC DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-COOH-EKT-HA-6HIS - C-TERMINAL 6 HIS AND HA DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-NH2-6HIS-GST-EKT - N-TERMINAL 6 HIS AND GST DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-NH2-6HIS-STREP-EKT - N-TERMINAL 6 HIS AND STREP DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-COOH-EKT-STAG-6HIS - C-TERMINAL 6 HIS AND STAG DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-NH2-6HIS-MBP-EKT - N-TERMINAL 6 HIS AND MBP DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-COOH-EKT-DAGFP-6HIS - C-TERMINAL 6 HIS AND DAGFP DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-COOH-EKT-FLUC-6HIS - C-TERMINAL 6 HIS AND FLUC DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-NH2-6HIS-BETAGAL-EKT - N-TERMINAL 6 HIS AND BETA GAL DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-COOH-EKT-BETAGAL-6HIS - C-TERMINAL 6 HIS AND BETA GAL DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-COOH-EKT-CAT-6HIS - C-TERMINAL 6 HIS AND CAT DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-COOH-FXA-KRYFP-6HIS - C-TERMINAL 6 HIS AND YFP DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-NH2-6HIS-FRCFP-THR - N-TERMINAL 6 HIS AND CFP DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-COOH-THR-RLUC-6HIS - C-TERMINAL 6 HIS AND RLUC DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-COOH-TEV-CMYC-6HIS - C-TERMINAL 6 HIS AND CMYC DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-NH2-6HIS-HA-TEV - N-TERMINAL 6 HIS AND HA DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-COOH-TEV-T7-6HIS - C-TERMINAL 6 HIS AND T7 DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-NH2-6HIS-HA-3C - N-TERMINAL 6 HIS AND HA DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-NH2-6HIS-STREP-3C - N-TERMINAL 6 HIS AND STREP DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-COOH-3C-MBP-6HIS - C-TERMINAL 6 HIS AND MBP DUAL TAG MAMMALIAN PLASMID PSF-CMV-PURO-NH2-FLAG?-FXA - N-TERMINAL FLAG? TAG MAMMALIAN PLASMID PSF-CMV-PURO-COOH-FXA-CMYC - C-TERMINAL CMYC TAG MAMMALIAN PLASMID PSF-CMV-PURO-COOH-FXA-GST - C-TERMINAL GST TAG MAMMALIAN PLASMID PSF-CMV-PURO-COOH-FXA-MBP - C-TERMINAL MBP TAG MAMMALIAN PLASMID PSF-CMV-PURO-NH2-CMYC-THR - N-TERMINAL CMYC TAG MAMMALIAN PLASMID PSF-CMV-PURO-NH2-HA-THR - N-TERMINAL HA TAG MAMMALIAN PLASMID PSF-CMV-BETAGAL - CMV BETA GALACTOSIDASE PLASMID Bacterial Strep Tag Vector Set Mammalian MBP Tag Vector Set Bacterial MBP Tag Vector Set Origin of Replication Vector Set Bacterial N-terminal Tag Vector Set Bacterial C-terminal Tag Vector Set Mammalian 10His Tag Vector Set Bacterial 10His Tag Vector Set Mammalian V5 Tag Vector Set