Bicine
- CAS No.
- 150-25-4
- Chemical Name:
- Bicine
- Synonyms
- BICINE BUFFER;N,N-BIS(2-HYDROXYETHYL)GLYCINE;BIClNE;BICINE;Biobuffer;BICINE, 99+%;Fe-3-Specific;Bicine, ULTROL;BICINE BIOXTRA;DIETHYLOLGLYCINE
- CBNumber:
- CB8470568
- Molecular Formula:
- C6H13NO4
- Molecular Weight:
- 163.17
- MDL Number:
- MFCD00004295
- MOL File:
- 150-25-4.mol
- MSDS File:
- SDS
| Melting point | 190 °C (dec.)(lit.) |
|---|---|
| Boiling point | 290.25°C (rough estimate) |
| Density | 1.05 g/mL at 20 °C |
| vapor pressure | 0.002-0.007Pa at 20-25℃ |
| refractive index | 1.4240 (estimate) |
| storage temp. | room temp |
| solubility | H2O: 1 M at 20 °C, clear, colorless |
| form | Powder |
| color | White |
| PH | 4.0-5.5 (25℃, 1M in H2O) |
| pka | 8.3(at 25℃) |
| PH Range | 7.6 - 9.0 |
| Water Solubility | soluble |
| λmax |
λ: 260 nm Amax: 0.070 λ: 280 nm Amax: 0.040 |
| Merck | 14,1201 |
| BRN | 1769362 |
| LogP | 2.3 at 20℃ and pH7 |
| Surface tension | 72.2mN/m at 1g/L and 20℃ |
| CAS DataBase Reference | 150-25-4(CAS DataBase Reference) |
| FDA UNII | 1J484QFI1O |
| EPA Substance Registry System | Bicine (150-25-4) |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS07 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal word | Warning | |||||||||
| Hazard statements | H302-H315-H319-H332-H335 | |||||||||
| Precautionary statements | P261-P280-P305+P351+P338 | |||||||||
| Hazard Codes | Xn | |||||||||
| Risk Statements | 20/21/22-36/37/38 | |||||||||
| Safety Statements | 24/25-36-26 | |||||||||
| WGK Germany | 3 | |||||||||
| RTECS | MB9700000 | |||||||||
| F | 3-10 | |||||||||
| TSCA | Yes | |||||||||
| HS Code | 29225000 | |||||||||
| Toxicity | mouse,LD50,intraperitoneal,1540mg/kg (1540mg/kg),KIDNEY, URETER, AND BLADDER: "CHANGES IN TUBULES (INCLUDING ACUTE RENAL FAILURE, ACUTE TUBULAR NECROSIS)",Revue d'Epidemiologie, Medecine Sociale et Sante Publique. Vol. 10, Pg. 391, 1962. | |||||||||
| NFPA 704 |
|
Bicine price More Price(70)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | 391336-M | Bicine, ULTROL Grade - CAS 150-25-4 - Calbiochem A zwitterionic amino acid buffer active in the pH 7.6-9.0 range. Has a pKa of 8.26 at 25°C. Absorbance (5%, H2O, 250 nm):0.05. | 150-25-4 | 100g | $159 | 2024-03-01 | Buy |
| Sigma-Aldrich | 391336-M | Bicine, ULTROL Grade - CAS 150-25-4 - Calbiochem A zwitterionic amino acid buffer active in the pH 7.6-9.0 range. Has a pKa of 8.26 at 25°C. Absorbance (5%, H2O, 250 nm):0.05. | 150-25-4 | 1kg | $907 | 2024-03-01 | Buy |
| Sigma-Aldrich | RES1151B-B7 | Bicine PharmaGrade, Manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production., suitable for cell culture | 150-25-4 | 1pkg | $157 | 2022-05-15 | Buy |
| Sigma-Aldrich | 391336-M | Bicine, ULTROL® Grade A zwitterionic amino acid buffer active in the pH 7.6-9.0 range. Has a pKa of 8.26 at 25°C. Absorbance (5%, H2O, 250 nm):0.05. | 150-25-4 | 391336-100GM | $136 | 2022-05-15 | Buy |
| Sigma-Aldrich | 391336-M | Bicine, ULTROL® Grade A zwitterionic amino acid buffer active in the pH 7.6-9.0 range. Has a pKa of 8.26 at 25°C. Absorbance (5%, H2O, 250 nm):0.05. | 150-25-4 | 391336-1KG | $775 | 2022-05-15 | Buy |
Bicine Chemical Properties,Uses,Production
Description
Bicine is an amino acid that results from amine degradation due to the presence of oxygen and/or sulfur dioxide. The amine solvents that undergo this degradation are MDEA, DEA, TEA, and mixed amine solvents containing any of these amines. As a zwitterionic amino acid buffer agent, bicine is active in the pH 7.6-9.0 range (pKa of 8.26 at 25°C). Bicine is a recommended buffer for low-temperature biochemical work. It is used for the preparation of stable substrate solution for serum guanase determination. It can be used as a nitrogenous strength enhancer to regulate cement hydration and improve cement properties. Studies have found that bicine significantly changes the morphology of portlandite crystals from a parallel-stacked lamellar shape to a distorted one and also decreases the pore size of the hardened cement paste at an early age[1]. In particular, bicine could promote the rapid formation of β-sheet-rich amyloid-β fibrils[2].
Chemical Properties
Bicine is a white/clear crystalline powder that is somewhat soluble in water. It contains an amino group, carboxyl group, and two hydroxyl units in its structure, making it highly polar. It is often used as a biological buffer and chelating agent.
Uses
To prepare stable substrate solution for the determination of guanine deaminase. The buffer is widely used with the working concentration of 3-100mM.
Uses
A zwitterionic amino acid buffer agent used for low temperature work. The use of bicine in a thin layer ion exchange chromatography method for protein resolution has been published. Bicine has been utilized in peptide and protein crystallization. A kinetic study of a quaternary transition-state analogue complex of creatine kinase used bicine in the reaction buffer. A multiphasic buffer system for SDS-PAGE of proteins and peptides that incorporates bicine has been described.
Definition
ChEBI: N,N-bis(2-hydroxyethyl)glycine is a bicine that is a Good's buffer substance, pKa = 8.35 at 20 ℃. It is a conjugate acid of a [bis(2-hydroxyethyl)amino]acetate. It is a tautomer of a [bis(2-hydroxyethyl)ammonio]acetate.
Purification Methods
Dissolve bicine in a small volume of hot water and precipitate it with EtOH, twice. Repeat once more but treat the aqueous solution with charcoal Purification of Biochemicals — Amino Acids and Peptides and filter before adding EtOH. Also crystallise it from concentrated aqueous solutions. [Torn & Kolthoff J Am Chem Soc 77 2061 1955, Chaberek et al. J Am Chem Soc 75 2185 1953, Beilstein 4 IV 2390.]
References
[1] Lu X, et al. The influence of bicine on the hydration and properties of Portland cement. Journal of Thermal Analysis and Calorimetry, 2022; 147: 13125–13134.
[2] Kim H, et al. Bicine promotes rapid formation of β-sheet-rich amyloid-β fibrils. PLOS ONE, 2020.
Bicine Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Suzhou Genemill Biotechnology Co.,Ltd | +8615862502491 | aimeegao@genemill.com | China | 39 | 58 |
| Hebei Weibang Biotechnology Co., Ltd | +8615531157085 | abby@weibangbio.com | China | 8811 | 58 |
| Hebei Mojin Biotechnology Co., Ltd | +86 13288715578 +8613288715578 | sales@hbmojin.com | China | 12840 | 58 |
| Hebei Chuanghai Biotechnology Co,.LTD | +86-13131129325 | sales1@chuanghaibio.com | China | 5893 | 58 |
| Shanghai Daken Advanced Materials Co.,Ltd | +86-371-66670886 | info@dakenam.com | China | 18777 | 58 |
| Henan Tianfu Chemical Co.,Ltd. | +86-0371-55170693 +86-19937530512 | info@tianfuchem.com | China | 21634 | 55 |
| Shanghai Time Chemicals CO., Ltd. | +86-021-57951555 +8617317452075 | jack.li@time-chemicals.com | China | 1803 | 55 |
| Hangzhou FandaChem Co.,Ltd. | +8615858145714 | FandaChem@Gmail.com | China | 9214 | 55 |
| AB PharmaTech,LLC | 323-480-4688 | sales@acrospharmatech.com | United States | 989 | 55 |
| career henan chemical co | +86-0371-86658258 +8613203830695 | sales@coreychem.com | China | 29882 | 58 |
View Lastest Price from Bicine manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2024-11-22 | Bicine
150-25-4
|
US $0.00 / Kg/Drum | 1KG | 99%min | 500kgs | WUHAN FORTUNA CHEMICAL CO., LTD | |
 |
2024-11-19 | Bicine
150-25-4
|
US $30.00-80.00 / mg | ≥98% | 10g | TargetMol Chemicals Inc. | ||
 |
2024-11-19 | Bicine
150-25-4
|
US $25.00 / ASSAYS | 100ASSAYS | 99.5% | 100 mt | Hebei Weibang Biotechnology Co., Ltd |
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