Description
Base treatment of human seminal plasma extracts converts the 19-hydroxy PGE compounds present in the sample into the corresponding PGB compounds generating strong conjugated dienone chromophores. This method has been employed to provide a rapid, UV-based analytical method for PGE quantitation. Due to its chemical stability and strong UV absorbance at 278 nm, 19(R)-hydroxy PGB2 is also used as an internal standard in extractions and HPLC to determine recovery of arachidonic acid metabolites.1,2,3
References
1. Antoine, C., Murphy, R.C., Henson, P.M., et al. Time-dependent utilization of platelet arachidonic acid by the neutrophil in formation of 5-lipoxygenase products in platelet-neutrophil co-incubations Biochim. Biophys. Acta 1128,139-146(1992).
2. Borgeat, P., and Picard, S. 19-Hydroxyprostaglandin B2 as an internal standard for on-line extraction-high-performance liquid chromatography analysis of lipoxygenase products Anal. Biochem. 171,283-289(1988).
3. Surette, M.E., Odeimat, A., Palmantier, R., et al. Reverse-phase high-performance liquid chromatography analysis of arachidonic acid metabolites in plasma after stimulation of whole blood ex vivo Anal. Biochem. 216(2),392-400(1994).
