MUNG BEAN NUCLEASE
MUNG BEAN NUCLEASE price More Price(3)
Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
---|---|---|---|---|---|---|---|
Sigma-Aldrich(India) | E8263 | Benzonase® Nuclease, ultrapure ≥250?units/μL, ≥99% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, ultrapure grade | 9025-65-4 | 5000UNITS | ₹18914.4 | 2022-06-14 | Buy |
Sigma-Aldrich(India) | T4330 | Turbonuclease from Serratia marcescens recombinant, expressed in E. coli | 9025-65-4 | 50000UNITS | ₹31934.7 | 2022-06-14 | Buy |
Sigma-Aldrich(India) | E8263 | Benzonase® Nuclease, ultrapure ≥250?units/μL, ≥99% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, ultrapure grade | 9025-65-4 | 25000UNITS | ₹63270 | 2022-06-14 | Buy |
MUNG BEAN NUCLEASE Chemical Properties,Uses,Production
Uses
The enzyme has been used for an assay to detect the amount of Tf1 RNA that is protected from degradation by the nuclease, Benzonase. The Tf1 element of Schizosaccharomyces pombe is a long terminal repeat-containing retrotransposon that encodes functional protease, reverse transcriptase, and integrase proteins. It has also been used in the fractionation of nuclear pellet and nuclear extract obtained from cell lysates.
General Description
Endonuclease from Serratia marcescens is a dimer containing two identical monomeric units with distinct protein folds. The core contains a six-stranded antiparallel β-sheet flanked by α-helices on either side. Each monomer bears one active site. This enzyme is a magnesium-dependent nucleases.
Biochem/physiol Actions
Benzonase? is a genetically engineered endonuclease from Serratia marcescens. The protein is a dimer of 30 kDa subunits with two essential disulfide bonds. This endonuclease attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of operating conditions. It completely digests nucleic acids to 5′- monophosphate terminated oligonucleotides 3 to 5 bases in length. This is ideal for the removal of nucleic acids from recombinant proteins. It can also be used for applications where complete digestion of nucleic acids is desirable. It also reduces viscosity in protein extracts and prevents cell clumping. Pre-treatment of a protein sample with this enzyme improves its resolution on 2D gel electrophoresis by eliminating any bound nucleic acids. The optimum pH for enzyme activity is found to be 8.0-9.2.
MUNG BEAN NUCLEASE Preparation Products And Raw materials
Raw materials
Preparation Products
MUNG BEAN NUCLEASE Suppliers
Supplier | Tel | Country | ProdList | Advantage | Inquiry |
---|---|---|---|---|---|
career henan chemical co | +86-0371-86658258 +8613203830695 | China | 29826 | 58 | Inquiry |
Henan Alfa Chemical Co., Ltd | +8618339805032 | China | 13096 | 58 | Inquiry |
Hzymes Biotechnology Co. LTD | +86-02160879177 +86-13849862354 | China | 116 | 58 | Inquiry |
SUZHOU SENFEIDA CHEMICAL CO.,LTD | +86-0512-83500002 +8615195660023 | China | 10814 | 58 | Inquiry |
LEAPCHEM CO., LTD. | +86-852-30606658 | China | 43348 | 58 | Inquiry |
Aladdin Scientific | +1-+1(833)-552-7181 | United States | 57511 | 58 | Inquiry |
Shanghai Aladdin Biochemical Technology Co.,Ltd. | +86-18521732826 | China | 48467 | 58 | Inquiry |
Shanghai Yingxin laboratory equipment Co., Ltd | 021-021-59178156 15300768757 | China | 10678 | 58 | Inquiry |
Hubei wei shi reagent group ltd., company | 027-02759101766 13125137661 | China | 4072 | 58 | Inquiry |
ACROBiosystems | 18514007688 | China | 971 | 58 | Inquiry |
9025-65-4(MUNG BEAN NUCLEASE)Related Search:
1of4
chevron_right