MUNG BEAN NUCLEASE

MUNG BEAN NUCLEASE Structure
CAS No.
9025-65-4
Chemical Name:
MUNG BEAN NUCLEASE
Synonyms
NUCLEASE;BENZONASE;NUCLEASE R;Binuclease;EC 3.1.30.1;EC 3.1.30.2;S1 NUCLEASE;NUCLEASE S1;NUCLEASE P1;ENDONUCLEASE
CBNumber:
CB4664699
Molecular Formula:
Molecular Weight:
0
MOL File:
Mol file
Modify Date:
2024/7/2 8:55:09

MUNG BEAN NUCLEASE Properties

storage temp. -20°C
form lyophilized powder
color Colorless to light yellow

SAFETY

Risk and Safety Statements

Safety Statements  23-24/25
WGK Germany  3
10-21

MUNG BEAN NUCLEASE price More Price(3)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich(India) E8263 Benzonase® Nuclease, ultrapure ≥250?units/μL, ≥99% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, ultrapure grade 9025-65-4 5000UNITS ₹18914.4 2022-06-14 Buy
Sigma-Aldrich(India) T4330 Turbonuclease from Serratia marcescens recombinant, expressed in E. coli 9025-65-4 50000UNITS ₹31934.7 2022-06-14 Buy
Sigma-Aldrich(India) E8263 Benzonase® Nuclease, ultrapure ≥250?units/μL, ≥99% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, ultrapure grade 9025-65-4 25000UNITS ₹63270 2022-06-14 Buy
Product number Packaging Price Buy
E8263 5000UNITS ₹18914.4 Buy
T4330 50000UNITS ₹31934.7 Buy
E8263 25000UNITS ₹63270 Buy

MUNG BEAN NUCLEASE Chemical Properties,Uses,Production

Uses

The enzyme has been used for an assay to detect the amount of Tf1 RNA that is protected from degradation by the nuclease, Benzonase. The Tf1 element of Schizosaccharomyces pombe is a long terminal repeat-containing retrotransposon that encodes functional protease, reverse transcriptase, and integrase proteins. It has also been used in the fractionation of nuclear pellet and nuclear extract obtained from cell lysates.

General Description

Endonuclease from Serratia marcescens is a dimer containing two identical monomeric units with distinct protein folds. The core contains a six-stranded antiparallel β-sheet flanked by α-helices on either side. Each monomer bears one active site. This enzyme is a magnesium-dependent nucleases.

Biochem/physiol Actions

Benzonase? is a genetically engineered endonuclease from Serratia marcescens. The protein is a dimer of 30 kDa subunits with two essential disulfide bonds. This endonuclease attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of operating conditions. It completely digests nucleic acids to 5′- monophosphate terminated oligonucleotides 3 to 5 bases in length. This is ideal for the removal of nucleic acids from recombinant proteins. It can also be used for applications where complete digestion of nucleic acids is desirable. It also reduces viscosity in protein extracts and prevents cell clumping. Pre-treatment of a protein sample with this enzyme improves its resolution on 2D gel electrophoresis by eliminating any bound nucleic acids. The optimum pH for enzyme activity is found to be 8.0-9.2.

MUNG BEAN NUCLEASE Preparation Products And Raw materials

Raw materials

Preparation Products

MUNG BEAN NUCLEASE Suppliers

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endonuclease serratia marcescens,*recombinant 90+ BENZONASE BENZONASE(R) BENZON NUCLEASE BENZONASE(R) NUCLEASE EC 3.1.30.2 EC 3.1.30.1 ENDONUCLEASE ENDONUCLEASE S1 (ASPERGILLUS ORYZAE) IUB: 3.1.30.1 S1 NUCLEASE NUCLEASE, MUNG BEAN NUCLEASE-GAMMA NUCLEASE NUCLEASE S1 (ASPERGILLUS ORYZAE) NUCLEASE S1 NUCLEASE R NUCLEASE P1 MUNG BEAN NUCLEASE BENZONASE (R) NUCLEASE, RECOMBINANT EXPRESSED IN ESCHERICHIA COLI BENZONASE (R) NUCLEASE, ULTRAPURE GRADE, RECOMBINANT EXPRESSED IN ESCHERICHIA COLI NUCLEASE FROM MUNG BEAN SPROUTS(PHASEOLUS AUREUS) NUCLEASE FROM MUNG BEAN SPROUTS benzonase, ultrapure endonuclease from serratia marcescens Benzonase(R) Nuclease, ultrapure Turbonuclease Benzonase? Nuclease, ultrapure Turbonuclease from Serratia marcescens Nuclease from Phaseolus aureus sprouts from mung bean Benzonase(R) Nuclease BENZONASE NUCLEASE Endonuclease Ⅰ Nuclease, nucleateendo- Nuclease(Endonuclease from Serratia marcescens) Ultra Nuclease( GMP class ) Benz-Neburase Binuclease Binuclease Liquid Binuclease, Benzonase Nuclease Broad spectrum Nuclease UltraNuclease 9025-65-4 BioChemical Application Index Biochemicals and Reagents Cell Lysis and Protein Extraction Reagents Metabolomics Nucleotide Metabolism Protein Sample Clarification Protein & Nucleic Acid Isolation Proteomics Proteomics and Protein Expression Sample Preparation General Metabolic Enzymes 3.1.x.x Acting on esters 3.x.x.x Hydrolases Enzymes, Inhibitors, and Substrates