Bovine albumin
- CAS No.
- 9048-46-8
- Chemical Name:
- Bovine albumin
- Synonyms
- Bovine serum albumin;AlbuMin Bovine V;BSAV;SERUM ALBUMIN;compound amino acid;Almond protein powder;ALBUMIN, BOVINE (BSA), CRYSTALLINE;nhsa;AC-BSA;Bovine SeruM AlbuMin solution
- CBNumber:
- CB5107573
- Molecular Formula:
- N/A
- Molecular Weight:
- 0
- MOL File:
- Mol file
- MSDS File:
- SDS
- Modify Date:
- 2024/7/2 9:23:19
RTECS | MT6446000 |
---|---|
storage temp. | 2-8°C |
solubility | PBS: >40 mg/mL |
form | lyophilized powder |
color | clear clear, colorless |
PH | 6.5-7.5 (1% in 0.15 M sodium chloride) |
Water Solubility | Soluble in water. |
Merck | 13,8542 |
Stability | Stable. Incompatible with strong oxidizing agents and strong acids. Refrigerate. |
EPA Substance Registry System | Albumins, blood serum (9048-46-8) |
SAFETY
Risk and Safety Statements
Symbol(GHS) | GHS07,GHS08 |
---|---|
Signal word | Danger |
Hazard statements | H302+H312+H332-H315-H319-H334-H335 |
Precautionary statements | P261-P264-P280-P301+P312-P302+P352+P312-P305+P351+P338 |
Hazard Codes | Xn |
Risk Statements | 22-52/53-21/22 |
Safety Statements | 24/25-61-36/37 |
WGK Germany | 3 |
F | 10-21 |
TSCA | Yes |
HS Code | 35029000 |
Toxicity | LD50 intravenous in monkey: > 12500mg/kg |
Bovine albumin price More Price(303)
Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
---|---|---|---|---|---|---|---|
Sigma-Aldrich(India) | P0914 | Protein standard Micro Standard, liquid | 9048-46-8 | 5AMP | ₹5233.8 | 2022-06-14 | Buy |
Sigma-Aldrich(India) | P0914 | Protein standard Micro Standard, liquid | 9048-46-8 | 10AMP | ₹9101.2 | 2022-06-14 | Buy |
Sigma-Aldrich(India) | B8894 | Albumin, Acetylated from bovine serum protease free, for molecular biology | 9048-46-8 | 0.5ML | ₹8784 | 2022-06-14 | Buy |
Sigma-Aldrich(India) | P0834 | Protein standard liquid, 2 mg protein/ml | 9048-46-8 | 10X1ML | ₹17812 | 2022-06-14 | Buy |
Sigma-Aldrich(India) | B8894 | Albumin, Acetylated from bovine serum protease free, for molecular biology | 9048-46-8 | 1.5ML | ₹21472 | 2022-06-14 | Buy |
Bovine albumin Chemical Properties,Uses,Production
Description
Bovine serum albumin is a protein which has a key role in regulating blood colloidal osmotic pressure. A water-soluble protein composed of 583 amino acids, BSA has a calculated molecular weight of 66,430 Daltons. Six α-helices form three homologous domains of BSA. Depending on pH, BSA undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.
This BSA product is specifically evaluated and tested for the following properties:
Very low protease content / protease-free
Very low fatty acid content / fatty acid-free
Protease-free BSA is especially useful for applications like:
Enzyme assays
Protein-based assays
Protease-sensitive techniques that include:
a. Enzyme immunoassay (EIA)
b. Nucleic acid hybridization
c. Radioimmunoassay (RIA)
Chemical Properties
Crystalline powder
Uses
Suitable for cell culture media supplement.
Bovine serum albumin (BSA) Fraction V has uses in many applications such as stabilizing proteins in solution, blocking non-specific binding in immuno-assays, and as a protein standard in various protein assays.
Stabilizer for proteins and enzymes in solution
Blocking agent for immuno-assays (ie, western blot, IHC, IF)
Minimizes non-specific antibody binding
IgG- and protease-free
50 g of solid powder
Bovine serum albumin (BSA) is commonly used as a stabilizing agent for proteins and enzymes, including dilute solutions of antibody. It is also used as a blocking agent to reduce non-specific antibody binding in immuno-detection procedures such as western blotting, immunofluorescence, and IHC. It is also suitable for use in protease sensitive assays such as RIA and EIA, as well as nucleic acid hybridization. This BSA is IgG- and protease-free.
Application
Bovine Serum Albumin has been used:
as a constituent of medium used for the differentiation of human pluripotent stem cells
in enzyme-linked immunosorbent assay (ELISA) and sandwich ELISA
to permeabilize fetal cortical cells
This Sigma A7030 BSA has specifically been used as a component of blocking buffer in a peptide-based ELISA assay for analysis of COVID-19 / SARS-CoV-2 samples (Poh, C.K. et al., Nat. Commun., 11(1), 2806 (2020)).
Definition
The word bovine means “cow,” and bovine serum albumin (BSA) is a protein that comes from cows. In particular, BSA is a type of protein called albumin, which is found in large amounts in the blood of cows. Blood is a mixture of red blood cells, which is why blood looks red, and many different types of proteins that are dissolved in water. If the cells in blood were removed, you would be left with a clear liquid, called serum. Thus, BSA is the albumin protein that is found in the blood of cows. In the blood, the job of albumin is to carry other proteins from place to place.
General Description
Bovine serum albumin (BSA) has an important role in the regulation of blood colloidal osmotic pressure. BSA is the major protein in bovine serum, and is composed of 583 amino acids with a calculated molecular weight of 66,430 Daltons. BSA is used in many different applications and fields, such as:
- Protein quantitation (as a standard)
- Immunochemistry (as a blocking agent)
- Cell culture (as a nutrient)
This particular BSA is specifically evaluated and tested to have very low protease content / for protease-free status. Protease-free BSA may be used in applications like:
- Enzyme assays
- Protein-based assays
- Protease-sensitive techniques that include:
b) Nucleic acid hybridization
c) Radioimmunoassay (RIA)
Biological Activity
Bovine Serum Albumin is a BSA (bovine serum albumin) is a widely used blocking reagent for use in immunohistochemistry (IHC), Immunocytochemistry (ICC), ELISAs and Western blotting. Used to block non-specific binding of antibodies. Can also be used as a protein concentration standard in Bradford assay for protein quantification. BSA is also used in cell culture.
Bovine albumin Preparation Products And Raw materials
Supplier | Tel | Country | ProdList | Advantage | Inquiry |
---|---|---|---|---|---|
ADVENT CHEMBIO PRIVATE LIMITED | +91-9821524116 +91-9821524116 | Mumbai, India | 353 | 58 | Inquiry |
JSK Chemicals | +919879767970 | Gujarat, India | 3756 | 58 | Inquiry |
TCI Chemicals (India) Pvt. Ltd. | 1800 425 7889 | New Delhi, India | 6778 | 58 | Inquiry |
ALPHA CHEMIKA | +91-22-22061123 +91-22-66382501 | Mumbai, India | 1681 | 43 | Inquiry |
Scientific OEM | +91-22- 2343 7546 / 2341 3094 | New Delhi, India | 1996 | 38 | Inquiry |
Otto Chemie Pvt. Ltd. | +91 9820041841 | Mumbai, India | 5873 | 58 | Inquiry |
Sisco Research Laboratories Pvt. Ltd. | +91-22-4268 5800 | Mumbai, India | 4317 | 58 | Inquiry |
Alfa Aesar | 1 800 209 7001 | Maharashtra, India | 6913 | 58 | Inquiry |
Triveni chemicals | 08048762458 | New Delhi, India | 6093 | 58 | Inquiry |
Central Drug House(P) Ltd. | 91-11-49404040 | New Delhi, India | 6160 | 58 | Inquiry |
Supplier | Advantage |
---|---|
ADVENT CHEMBIO PRIVATE LIMITED | 58 |
JSK Chemicals | 58 |
TCI Chemicals (India) Pvt. Ltd. | 58 |
ALPHA CHEMIKA | 43 |
Scientific OEM | 38 |
Otto Chemie Pvt. Ltd. | 58 |
Sisco Research Laboratories Pvt. Ltd. | 58 |
Alfa Aesar | 58 |
Triveni chemicals | 58 |
Central Drug House(P) Ltd. | 58 |
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