ABTS
- CAS No.
- Chemical Name:
- ABTS
- Synonyms
- ABTS;ABTS(R);ABTS ENHANCER;ABTS(TM) CHROMOPHORE;2,2'-AZINO-DI-(3-ETHYLBENZTHIAZOLINESULPHONIC ACID);2,2'-AZINO-BIS(3-ETHYLBENZTHIAZOLINE-6-SULFONIC ACID);2,2'-AZIDO-BIS[3-ETHYLBENZTHIAZOLINE-6-SULFONIC ACID];2,2'-AZINO-BIS(3-ETHYLBENZOTHIAZOLINE-6-SULFONIC ACID);2,2'-AZINO-DI-(3-ETHYLBENZ- THIAZOLINE-6-SULFONIC ACID);2,2'-AZINO-BIS(3-ETHYLBENZOTHIAZOLINE-6-SULFONIC ACID) ENHANCER
- CBNumber:
- CB7682263
- Molecular Formula:
- C18H18N4O6S4
- Molecular Weight:
- 514.62
- MOL File:
- Mol file
ABTS Chemical Properties,Uses,Production
Uses
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) has been used as a peroxidase substrate in ELISA experiment. This substrate gives a green color soluble end product. It has also been used as a colloidal preparation spread over the standard media plates, to evaluate the lignin oxidation activity. Presence of green coloration of the substrate exhibits microbial laccase activity.
General Description
The product is supplied as a ready-to-use one component substrate for peroxidase consisting 2,2′-azino-bis (3-ethylbenz-thiazoline-6-sulfonic acid) in an acidic buffer. The substrate is colorless before reacting with peroxidase and produces a blue-green color reaction product after reacting with it. 1% sodium dodecyl sulfate (SDS) may be used to stop the reaction during end-point assays. Since this substrate produces a soluble reaction product, it is not recommended for immunoblotting or histochemical applications.
Biochem/physiol Actions
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) is a cation and undergoes oxidation in the presence of hydrogen peroxide. The oxidized ABTS is a blue-green product and is measurable colorimetrically. A combination of ABTS, hydrogen peroxide and myoglobin is a standard system employed for measuring total antioxidant capacity(TAC) from samples. ABTS method is flexible at varying pH and most widely used. ABTS is a substrate for phenol oxidase enzyme.
ABTS Preparation Products And Raw materials
Raw materials
Preparation Products
ABTS Suppliers
| Supplier | Tel | Country | ProdList | Advantage | Inquiry |
|---|---|---|---|---|---|
| EMD Biosciences, Inc. | 858 450 5500 | United States | 6529 | 66 | Inquiry |
| VWR International | 800 932 5000 | United Kingdom | 6554 | 82 | Inquiry |
| BIOTREND Chemikalien GmbH | 49 (0) 221 949 83 20 | Germany | 6115 | 76 | Inquiry |
| INDOFINE Chemical Company, Inc. | 1 888 463 6346 | United States | 6177 | 69 | Inquiry |
| Zymed Laboratories Inc. | 800 874 4494 (Customer Service) | United States | 3068 | 86 | Inquiry |
| MP Biomedicals, Inc. | 949 833 2500 | United States | 6566 | 81 | Inquiry |
| AMRESCO Inc. | 800 448 4442 | United States | 760 | 73 | Inquiry |
| Rockland Immunochemicals Inc. | 800 656 ROCK | United States | 2043 | 76 | Inquiry |
| Chemicon Internatinal, Inc. | 951 676 8080 | United States | 4175 | 65 | Inquiry |
| Kanto Chemical Co., Inc. | +81 3 3663 7631 | Japan | 6762 | 74 | Inquiry |
| Supplier | Advantage |
|---|---|
| EMD Biosciences, Inc. | 66 |
| VWR International | 82 |
| BIOTREND Chemikalien GmbH | 76 |
| INDOFINE Chemical Company, Inc. | 69 |
| Zymed Laboratories Inc. | 86 |
| MP Biomedicals, Inc. | 81 |
| AMRESCO Inc. | 73 |
| Rockland Immunochemicals Inc. | 76 |
| Chemicon Internatinal, Inc. | 65 |
| Kanto Chemical Co., Inc. | 74 |