Uses
Cleavable succinimidyl ester/NHS functionalized cyclooctyne derivative for incorporation of the cyclooctyne moiety into amine containing compounds or biomolecules. Cyclooctynes are useful in strain-promoted copper-free azide-alkyne cycloaddition reactions. This dibenzocyclooctyne will react with azide functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage. The disulfide present can be readily cleaved using mild reducing conditions (10-50 mM DTT at 37°C for 30 min or 5% ?-mercaptoethanol in SDS-PAGE sample buffer at 100°C for 5 minutes).
Caution: Please avoid reducing agents and amine or azide containing buffers during coupling/labeling reactions. Only use reducing agents when cleavage of the disulfide is desired.
