アガロース 化学特性,用途語,生産方法
外観
白色～わずかにうすい褐色, 粉末
定義
本品は、紅藻 Gracilaria から抽出される多糖体である。
溶解性
水、エタノール及びジエチルエーテルにほとんど溶けないが、水中に放置すると徐々に水を吸収して膨張する。熱水に溶けやすく、その液は冷時、半透明のゲルとなる。
解説
アガロース，寒天の主成分である重合度20～60の多糖で，β-(1→4)-D-ガラクトシルα-(1→3)-L-3,6-アンヒドロガラクトースを繰返し単位とする．微粒子化したものはゲルクロマトグラフィーの担体として用いられる．熱水で溶かし，ゲル化したものは，ポリアクリルアミドゲルに比べ，大きな網目構造を有するので，核酸を電気泳動する際の支持体として利用されている．
森北出版「化学辞典（第2版）
用途
電気泳動の支持体。
化粧品の成分用途
保湿.湿潤剤、親水性増粘剤
説明
Agarose is the neutral polymer component of agar. Agar belongs to the group of galactopolysaccharides, which are the intracellular matrix of red algae/seaweeds of the Rhodophyta (red algae phylum). Agar is naturally slightly sulphated and is a complex mixture of polysaccharides. Agarose forms a solution when heated and condenses into a gel when cooled. Gel formation is due to the formation of a double helix of molecular chains, which creates a water-fixing network. Agarose is commonly used for the analysis of nucleic acids by gel electrophoresis or blotting (Northern or Southern), and is also suitable for protein analyses such as Ouchterlony and Radial Immunodiffusion (RID). Ethidium bromide and SYBR Green background staining is low.
化学的特性
Granular free flowing
使用
Agarose is frequently used in molecular biology for the separation of large molecules, especially DNA, by electrophoresis. Slabs of agarose gels (usually 0.7 - 2%) for electrophoresis are readily prepared by pouring the warm, liquid solution into a mold. A wide range of different agaroses, of varying molecular weights and properties are commercially available for this purpose. Used in high resolution protein electrophoresis, immunoelectrophoresis, electro immunoassays such as rocket assays, counter current electrophoresis.
定義
agarose: A carbohydrate polymerthat is a component of agar. It is usedin chromatography and electrophoresis.
一般的な説明
Agarose, a natural polymer is obtained from sea algae. This neutral linear polysaccharide is made up of?d-galactose and 3,6-anhydro-l-galactose. These are connected alternatively with the help of glycosidic bonds. Agarose has high significance in gel electrophoresis and gel chromatography.
农业用途
Agarose is a partially methylated neutral polysaccharide
used for making gel.
アガロース 上流と下流の製品情報
原材料
準備製品