バクテリオロドプシン HALOBACTERIUM SALINARUM由来 化学特性,用途語,生産方法
性質
7回膜貫通型.N末端はpyroGlu, Lys229でレチナールと結合.光プロトンポンプ機能
解説
高度好塩菌の一種から単離されたタンパク質で,レチナールを発色団とする.光照射によってレチナールが全trans型から11-cis型に変換することを利用してプロトンを細胞内から細胞外へ輸送する役割を果たしている膜タンパクで質ある.このプロトン駆動力を利用してATPが合成される.
天然物の起源
高度好塩菌(Halobacterium salinarum)紫膜:14~262
使用
Bacteriorhodopsin from
Halobacterium salinarum has been used:
- in generation of droplet lipid bilayer
- as a standard in quadrupole time-of-flight (QTOF) mass spectroscopy (MS)
- in the generation of protein-detergent complex and micelles for dynamic light scattering studies
定義
bacteriorhodopsin: A membraneboundprotein of the halophilic (saltresistant)bacterium Halobacteriumhalobium. When activated by light, itpumps protons out of the cell; thiscreates a concentration gradient,which enables ATP to be synthesized.Bacteriorhodopsin is composed ofseven α-helix segments, which spanthe membrane and are joined togetherby short amino-acid chains. Itcontains the prosthetic group retinal,which is also found in the pigmentrhodopsin in the rod cells of vertebrates.
一般的な説明
Bacteriorhodopsin (BR) is a covalent complex comprising bacterioopsin protein and retinal cofactor in the equimolar ratio. It corresponds to the molecular weight of 27kDa. BR belongs to the retinylidene class of proteins. It is a seven-membrane helical protein that acts as a photon-driven pump. BR can be used in studies of the folding and kenetics of β-helical proteins.
バクテリオロドプシン HALOBACTERIUM SALINARUM由来 上流と下流の製品情報
原材料
準備製品