Duolink In Situ Probemaker PLUS

Duolink In Situ Probemaker PLUS 価格 もっと(2)

メーカー	製品番号	製品説明	CAS番号	包装	価格	更新時間	購入
Sigma-Aldrich Japan	DUO92009	Duolink® In Situ Probemaker PLUS		1KIT	￥120000	2024-03-01	購入
Sigma-Aldrich Japan	DUO92009	Duolink? In Situ Probemaker PLUS		1kt	￥87900	2018-12-25	購入

Duolink In Situ Probemaker PLUS 化学特性,用途語,生産方法

使用

To perform a complete Duolink^? PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink^? PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.HRP is also available for brightfield detection.

Specificity
Duolink^? In Situ Probemaker extends your possibilities even further by allowing you to create your own PLA probes to meet specific assay requirements.

• Study homodimers
• Study protein-protein interactions with two primary antibodies derived from the same species
• Study protein-protein interactions with primary antibodies from any species

Using Duolink^? In Situ Probemaker PLUS and MINUS, you simply conjugate the PLUS and MINUS oligo arms directly to your antibodies in a quick and convenient procedure. As well as providing unique capabilities, this also means there is no longer any limitation as to which species your primary antibodies have to be derived from in order perform a Duolink^? In Situ assay.

Application Note
The antibody to be conjugated must have a concentration of 1 mg/ml. Do not use volumes larger than 20 ml for conjugation. The antibody must be in an amine free buffer, ideally PBS. The buffer should be carrier free but may contain up to 0.1% BSA, 5% trehalose, and 0.02% sodium azide.

• Study homodimers by using one monoclonal antibody split into two halves. Label one with the PLUS oligo and the other with the MINUS oligo.
• Use antibodies from same species: study protein-protein interactions, protein modifications, or single proteins, using two primary antibodies derived from the same species. Label one of the antibodies with the PLUS oligo and the other with the MINUS oligo
• Use antibodies from any species: study protein-protein interactions, protein modifications, or single proteins, using one or both primary antibodies derived from species other than mouse, rabbit or goat. Label one of your secondary antibodies with the PLUS oligo and the other with the MINUS oligo. Or, combine one labeled secondary antibody with a standard secondary Duolink^?In Situ PLA probe.

Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink^? in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink^? projects

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Duolink In Situ Probemaker PLUS 上流と下流の製品情報

原材料

準備製品

Duolink In Situ Probemaker PLUS 生産企業

名前	電話番号	電子メール	国籍	製品カタログ	優位度
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51471	80

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• Duolink? In Situ Probemaker PLUS