ANTI-CONNEXIN 43

ANTI-CONNEXIN 43 C화학적 특성, 용도, 생산

용도

Anti-Connexin-43 may be used in immunoblotting, immunocytochemistry and immunohistochemistry (frozen and formalin-fixed, paraffin-embedded tissues). Polyclonal antibodies reacting specifically with Cx43 may be applied in diverse cellular and molecular approaches to the study of gap junctions and their properties.
A minimum working dilution of 1:8,000 is determined by immunoblotting using a whole extract from mouse brain.
A minimum working dilution of 1:400 is determined by indirect immunofluorescent staining of acetone-fixed cultured baby hamster kidney (BHK).
A minimum working dilution of 1:2,000 is determined by indirect immunofluorescent staining of rat heart. (Negative on rat liver sections)
A minimum working dilution of 1:2,000 is determined by indirect immunoperoxidase staining of trypsin-digested,formalin-fixed, paraffin-embedded human or animal tissue.

일반 설명

Monoclonal Anti-Connexin-43 (mouse IgM isotype) is derived from the CXN-6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Connexin 43 (Cx43) is also known as GJA1 (gap junction protein alpha 1). The structure of connexin molecules includes a cytoplasmic N-terminal region, four transmembrane domains, two extracellular loops and a C-terminal cytoplasmic tail of varying length.

Biochem/physiol Actions

Connexin-43 (Cx-43) might play a vital role in neuronal migration as it resides in radial glial fibers which are closely associated with migrating neurons. It is also involved in transmembrane NAD+ transport. Connexin-43, which is an element of gap junction, facilitates cell-cell communication. Cx-43 is known to induce [Ca2+]i burst phenomenon in uterine artery endothelial cells (UAECs). Elevated expression of Cx-43 has been observed during early stages of human coronary atherosclerosis. Reduction or alteration in the levels or types of connexin expressed in various cell types correlates with tumor progression and metastasis. However, glioma cells transfected with the oncogene neu (c-erb-B2) exhibit a major reduction in intercellular communication with no decrease in overall expression of Cx43. Different states of rat Cx43 phosphorylation are found during development and lactation. Connexin 43 mutations occur in children whose hearts failed to develop asymmetry and lethal heart malformations occur on knocking out this gene in mice. Polyclonal antibodies reacting specifically with Cx438-11 may be applied in diverse cellular and molecular approaches to the study of gap junctions and their properties, and to correlate their expression pattern in a variety of cell types and tissues with physiological functions or pathological conditions.

ANTI-CONNEXIN 43 준비 용품 및 원자재

원자재

준비 용품

ANTI-CONNEXIN 43 공급 업체

공급자	전화	이메일	국가	제품 수	이점
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51471	80