플루오레세인
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플루오레세인 속성
- 녹는점
- 320 °C(lit.)
- 끓는 점
- 429.44°C (rough estimate)
- 밀도
- 1.2739 (rough estimate)
- 굴절률
- 1.5000 (estimate)
- 저장 조건
- room temp
- 용해도
- 용해도 물, 에테르, 벤젠, 클로로포름에 불용성; 에탄올, 메탄올, 아세톤, 에틸 아세테이트, N,N-디메틸포름아미드에 용해됨
- 색상 색인 번호
- 45350
- 물리적 상태
- 가루
- 산도 계수 (pKa)
- 2.2, 4.4, 6.7(at 25℃)
- 색상
- 빨간색에서 주황색으로
- pH 범위
- Pink uorescence (4.0) to green uorescence (6.0)
- 수용성
- 불용성
- 최대 파장(λmax)
- 493.5nm, 496nm, 460nm, 515nm
- Merck
- 14,4159
- BRN
- 94324
- 안정성
- 안정적인. 타기 쉬운. 강한 산화제와 호환되지 않습니다.
- 주요 응용
- Organic light-emitting diode, nanoparticles, liquid crystal display, oil products, lip make-up, nucleic acid synthesis, amplification, sequencing and cloning, diagnosis of diabetic retinopathy, detecting yeast, multidrug resistance, proteins, antiHCV antibodies, target genes, enzymatic activity, nerve agent, nucleic acid sequences, imaging lung cancer, prostate cancer
- 생물학 분야의 응용
- Detecting chromosomal aberration; diagnosing corneal diseases; marking/detecting insects; as antitumor agent; as antihistaminic agent; use in agriculture and plant cultivation; use in cosmetics; use in ophthalmology; as a substrate for measuring α-amylases activity, elastases activity, esterases activity, β-glucuronidases activity, horseradish peroxidases activity, kinases activity, monoamine oxidases (MAOs) activity, phosphatases activity, phospholipases activity, proteases/proteinases activity, telomerases activity activity
- LogP
- 2.980 (est)
- CAS 데이터베이스
- 2321-07-5(CAS DataBase Reference)
안전
- 위험 및 안전 성명
- 위험 및 사전주의 사항 (GHS)
위험품 표기 | Xi | ||
---|---|---|---|
위험 카페고리 넘버 | 43-36-36/37/38 | ||
안전지침서 | 22-24/25-37/39-26-36/37/39-27 | ||
WGK 독일 | 3 | ||
RTECS 번호 | LM5075000 | ||
F 고인화성물질 | 10-21 | ||
TSCA | Yes | ||
HS 번호 | 32042000 | ||
유해 물질 데이터 | 2321-07-5(Hazardous Substances Data) | ||
기존화학 물질 | KE-10869 |
플루오레세인 C화학적 특성, 용도, 생산
개요
Fluorescein is a synthetic organic compound available as a dark orange/red powder slightly soluble in water and alcohol. It is widely used as a fluorescent tracer for many applications.Fluorescein is a fluorophore commonly used in microscopy, in a type of dye laser as the gain medium, in forensics and serology to detect latent blood stains, and in dye tracing. Fluorescein has an absorption maximum at 494 nm and emission maximum of 521 nm (in water). The major derivatives are fluorescein isothiocyanate (FITC) and, in oligonucleotide synthesis, 6-FAM phosphoramidite.
Fluorescein also has an isosbestic point (equal absorption for all pH values) at 460 nm. Fluorescein is also known as a color additive (D&C Yellow no. 7). The disodium salt form of fluorescein is known as uranine or D&C Yellow no. 8.
The color of its aqueous solution varies from green to orange as a function of the way it is observed: by reflection or by transmission, as it can be noticed in bubble levels in which fluorescein is added as a colorant to the alcohol filling the tube to increase the visibility of the air bubble and the precision of the instrument. More concentrated solutions of fluorescein can even appear red.
화학적 성질
Orange-red, crystalline powder. Very dilute alkaline solutions exhibit intense greenishyellow fluorescence by reflected light, while the solution is reddish-orange by transmitted light.soluble in dilute alkalies, boiling alcohol, ether, dilute acids, and물리적 성질
The fluorescence of this molecule is very intense; peak excitation occurs at 494 nm and peak emission at 521 nm.Fluorescein has a pKa of 6.4, and its ionization equilibrium leads to pH-dependent absorption and emission over the range of 5 to 9. Also, the fluorescence lifetimes of the protonated and deprotonated forms of fluorescein are approximately 3 and 4 ns, which allows for pH determination from non intensity based measurements. The lifetimes can be recovered using time-correlated single photon counting or phase-modulation fluorimetry.
용도
Fluorescein is used as a fluorescent tracer for many applications including in a type of dye laser as the gain medium, in forensics and serology to detect latent blood stains and in dye tracing. It is used to localise multiple muscular ventricular septal defects during open heart surgery and confirm the presence of any residual defects. It is applied to teeth to reveal plaque.주요 응용
Biochemical researchIn cellular biology, the isothiocyanate derivative of fluorescein is often used to label and track cells in fluorescence microscopy applications (for example, flow cytometry). Additional biologically active molecules (such as antibodies) may also be attached to fluorescein, allowing biologists to target the fluorophore to specific proteins or structures within cells. This application is common in yeast display.
Health care applications
"Fluorescein sodium", the sodium salt of fluorescein, is used extensively as a diagnostic tool in the field of ophthalmology and optometry, where topical fluorescein is used in the diagnosis of corneal abrasions, corneal ulcers and herpetic corneal infections. It is also used in rigid gas permeable contact lens fitting to evaluate the tear layer under the lens.
Uses in river systems
In 1966, environmentalists forced a change to a vegetable-based dye to protect local wildlife. Other uses of fluorescein include using it as a water-soluble dye added to rainwater in environmental testing simulations to aid in locating and analyzing any water leaks, and in Australia and New Zealand as a methylated spirit dye.
Oil field application
Fluorescein dye solutions, typically 15 % active, are commonly used as an aid to leak detection during hydrostatic testing of sub sea oil and gas pipelines and other subsea infrastructure. Leaks can be detected by divers carrying ultraviolet lights.
정의
ChEBI: A xanthene dye that is highly fluorescent, detectable even when present in minute quantities. Used forensically to detect traces of blood, in analytical chemistry as an indicator in silver nitrate titrations and in microscopy.일반 설명
Yellow amorphous solid or orange-red crystals. Latter have greenish-yellow fluorescence by reflected light. Insoluble in water. Soluble in dilute aqueous bases. Very dilute alkaline solutions exhibit intense, greenish-yellow fluorescence by reflected light. Low toxicity. May be sensitive to prolonged exposure to light.공기와 물의 반응
Insoluble in water.반응 프로필
Fluorescein is incompatible with strong oxidizers. Also incompatible with acids, acid salts and salts of heavy metals. .화재위험
Flash point data for Fluorescein is not available, but Fluorescein is probably combustible.Safety Profile
Poison by intravenous route. Moderately toxic by intraperitoneal route. Mutation data reported. When heated to decomposition it emits acrid smoke and irritating fumes. See also FLUORESCEIN SODIUM.Safety
Topical, oral, and intravenous use of fluorescein can cause adverse reactions, including nausea, vomiting, hives, acute hypotension, anaphylaxis and related anaphylactoid reaction, causing cardiac arrest and sudden death due to anaphylactic shock.The most common adverse reaction is nausea, due to a difference in the pH from the body and the pH of the sodium fluorescein dye; a number of other factors , however, are considered contributors as well. The nausea usually is transient and subsides quickly. Hives can range from a minor annoyance to severe, and a single dose of antihistamine may give complete relief. Anaphylactic shock and subsequent cardiac arrest and sudden death are very rare, but because they occur within minutes, a health care provider who uses fluorescein should be prepared to perform emergency resuscitation.
플루오레세인 준비 용품 및 원자재
원자재
준비 용품
에리트로신 B
FLUORESCEIN CHLORIDE
2',7'-dibromo-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3-one
Fluorescein isothiocyanate
3',6'-디메톡시플루오란
EOSIN B
4'-Bromo-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
3',6'-Bis{[4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)oxy]-3H-[spiro(isobenzofuran-1,9,-xanthen]-3-one} FBBBE
플루오레세인이부티레이트
2-(3,7-DIHYDROXY-DIBENZO[B,F][1,4]OXAZEPIN-11-YL)-벤조산
등색206호
플루오레세인 공급 업체
글로벌( 416)공급 업체
공급자 | 전화 | 이메일 | 국가 | 제품 수 | 이점 |
---|---|---|---|---|---|
Shenyang Simchoice Chemical Co.,Ltd | +86-024-23769576 +86-15040101888 |
sales@simchoicechem.com | China | 255 | 58 |
Hebei Guanlang Biotechnology Co,.LTD | +8619930503252 |
daisy@crovellbio.com | China | 5828 | 58 |
Henan Fengda Chemical Co., Ltd | +86-371-86557731 +86-13613820652 |
info@fdachem.com | China | 20294 | 58 |
Hebei Saisier Technology Co., LTD | +86-18400010335 +86-18034520335 |
admin@hbsaisier.cn | China | 996 | 58 |
Hebei Longbang Technology Co., LTD | +86-18032476855 +86-18032476855 |
admin@hblongbang.com | China | 935 | 58 |
HebeiShuoshengImportandExportco.,Ltd | +86-18532138899 +86-18532138899 |
L18532138899@163.com | China | 931 | 58 |
Shanghai Daken Advanced Materials Co.,Ltd | +86-371-66670886 |
info@dakenam.com | China | 16914 | 58 |
Henan Tianfu Chemical Co.,Ltd. | +86-0371-55170693 +86-19937530512 |
info@tianfuchem.com | China | 21668 | 55 |
Hangzhou FandaChem Co.,Ltd. | 008657128800458; +8615858145714 |
fandachem@gmail.com | China | 9332 | 55 |
ATK CHEMICAL COMPANY LIMITED | +undefined-21-51877795 |
ivan@atkchemical.com | China | 32760 | 60 |