Proteinase K, recombinant, PCR Grade

Properties

Safety Information

Symbol(GHS):
Signal word:	Danger
Hazard statements:	Code Hazard statements Hazard class Category Signal word Pictogram P-Codes H317 May cause an allergic skin reaction Sensitisation, Skin Category 1 Warning P261, P272, P280, P302+P352,P333+P313, P321, P363, P501 H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled Sensitisation, respiratory Category 1 Danger P261, P285, P304+P341, P342+P311,P501
Precautionary statements:	P261 Avoid breathing dust/fume/gas/mist/vapours/spray. P280 Wear protective gloves/protective clothing/eye protection/face protection. P284 Wear respiratory protection. P304+P340 IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing. P333+P313 IF SKIN irritation or rash occurs: Get medical advice/attention. P342+P311 IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.

Description

• This PCR grade Proteinase K is extremely effective on native proteins and can therefore be used to rapidly inactivate endogenous nucleases such as RNases and DNases. This property makes Proteinase K particularly suitable for the isolation of native RNA and DNA from tissues or cell lines.
• The enzyme promotes cell lysis by activating a bacterial autolytic factor.
• Proteinase K is also used for the analysis of membrane structures by modifying proteins and glycoproteins on cell surfaces.
• The enzyme is particularly well suited for isolating nucleic acids for amplification reactions.
• Proteinase K can be used to remove cellular debris during the preparation of colony lifts, and to treat tissue sections to ensure efficient probe infiltration during in situ hybridization.