Anti-acetyl SMC3 Antibody (Lys105/106), clone 21A7
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- $401
- Product name: Anti-acetyl SMC3 Antibody (Lys105/106), clone 21A7
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- MW: 0
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- Synonyms:Anti-acetyl SMC3 Antibody (Lys105/106), clone 21A7
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Selected condition：
Brand
- Sigma-Aldrich
Package
- 100μL
- ManufacturerSigma-Aldrich
- Product numberMABE1073
- Product descriptionAnti-acetyl SMC3 Antibody (Lys105/106), clone 21A7 clone 21A7, from mouse
- Packaging100μL
- Price$401
- Updated2024-03-01
- Buy
| Manufacturer | Product number | Product description | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|
| Sigma-Aldrich | MABE1073 | Anti-acetyl SMC3 Antibody (Lys105/106), clone 21A7 clone 21A7, from mouse | 100μL | $401 | 2024-03-01 | Buy |
Properties
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Description
ELISA Analysis: A representative lot bound Lys105/106-diacetylated SMC3 peptide with 2-fold higher affinity than Lys105-monoacetylated SMC3 peptide (Kd = 0.04 and 0.09 μg/mL, respectively), while displaying much reduced affinity toward Lys105-monoacetylated (Kd = 0.73 μg/mL) and little affinity toward nonacetylated (Kd = 2.99 μg/mL) peptide (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected enhanced SMC3 occupancy at target chromatin sites in HDAC8-null HCT116 cells than wild-type HCT-116 cells (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).
ChIP-seq Analysis: A representative lot detected SMC3 chromosome 11 enrichment sites, including 81% of Rad21 target sites and 70% of Esco1 target sites, by ChIP-seq (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).
Western Blotting Analysis: A representative lot detected SMC3 Lys105/106 acetylation in HeLa cells. ESCO1 or ESCO2 depletion by shRNA treatment reduced SMC3 Lys105/106 acetylation level, while dual ESCO1/2 depletion resulted in most profound SMC3 Lys105/106 acetylation reduction (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).