| | EthidiuM-d5 BroMide Basic information More.. |
| Product Name: | EthidiuM-d5 BroMide | | Synonyms: | EthidiuM-d5 BroMide;2,7-DiaMino-10-
(ethyl-d5)-9-phenylphenanthridiniuM BroMide;2,7-DiaMino-9-phenyl-10-(ethyl-d5)
phenanthridiniuM BroMide;2,7-DiaMino-9-phenylphenanthridine-d5 EthobroMide;3,8-DiaMino-5-(ethyl-d5)-6-phenylphenanthridiniuM BroMide;DroMilac-d5;HoMidiuM-d5 BroMide | | CAS: | | | MF: | C21H20BrN3 | | MW: | 394.3076 | | EINECS: | | | Mol File: | Mol File |  |
Use
EthidiuM-d5 BroMide is the labelled ethidium bromide (EtBr). It is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). EthidiuM-d5 Bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.
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EthidiuM-d5 BroMide
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