Company Name: |
3B Pharmachem (Wuhan) International Co.,Ltd.
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Tel: |
821-50328103-801 18930552037 |
Email: |
3bsc@sina.com |
Products Intro: |
Product Name:DPN I CAS:81295-14-9 Purity:99% HPLC Package:1Mg ; 5Mg;10Mg ;100Mg;250Mg ;500Mg ;1g;2.5g ;5g ;10g
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Company Name: |
Sigma-Aldrich
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Tel: |
021-61415566 800-8193336 |
Email: |
orderCN@merckgroup.com |
Products Intro: |
Product Name:Dpn I CAS:81295-14-9 Purity:from Diplococcus pneumoniae Package:1EA Remarks:DPNI-RO
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| DPN I Chemical Properties |
storage temp. | -20°C | form | buffered aqueous glycerol solution |
RIDADR | UN3373 - class 6.2 Biological substance, Category B | WGK Germany | 3 |
| DPN I Usage And Synthesis |
Uses | In PCR-mediated mutagenesis, DpnI nuclease has been used for the digestion of wild type DNA after PCR. | General Description | Isoschizomers The enzyme is an isoschizomer to Bsp143 I, Dpn II, Mbo I, Nde II and Sau3A I. Methylation sensitivity The enzyme is only inhibited by the occurrence of 5-methylcytosine at the indicated site (*) if no 6-methyladenine is present. Typical ligation and recutting assay Dpn I fragments obtained by complete digestion of 1μg pBR322 DNA are ligated for 16 hours at +4°C with 1U T4 DNA Ligase in 10μl buffer that contains 66mM Tris-HCl, 5mM MgCl2, 5mM Dithiothreitol, 1mM ATP, pH 7.5 (at +20°C). The percentages of product that can be ligated and subsequently recut with Dpn I (yielding the typical pattern of pBR322 × Dpn I fragments) are stated under "Lig" and "Rec" in the certificate of analysis. |
| DPN I Preparation Products And Raw materials |
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