巴佛洛霉素C 1
| 中文名称 | 巴佛洛霉素C 1 |
|---|---|
| 中文同义词 | 巴佛洛霉素C 1;巴伐洛霉素 C1;巴弗洛霉素C1;巴菲霉素C1;化合物 T22598;巴非霉素C1 |
| 英文名称 | BAFILOMYCIN C1 |
| 英文同义词 | 2-Demethyl-2-methoxy-24-methylhygrolidin;Antibiotic L-681110A1;L-681110A1;BAFILOMYCIN C1;Bafilomycin C1, from Streptomyces griseus;2-Butenedioic acid (2E)-, 1-[(2R,4R,5S,6R)-tetrahydro-2-hydroxy-2-[(1S,2R,3S)-2-hydroxy-3-[(2R,3S,4E,6E,9S,10S,11R,12E,14Z)-10-hydroxy-3,15-dimethoxy-7,9,11,13-tetramethyl-16-oxooxacyclohexadeca-4,6,12,14-tetraen-2-yl]-1-methylbutyl]-5-methyl-6-(1-methylethyl)-2H-pyran-4-yl] ester;Bafilomycin C-1,Bafilomycin C1 |
| CAS号 | 88979-61-7 |
| 分子式 | C39H60O12 |
| 分子量 | 720.89 |
| EINECS号 | |
| 相关类别 | 微生物代谢物 |
| Mol文件 | 88979-61-7.mol |
| 结构式 |  |
巴佛洛霉素C 1 性质
| 储存条件 | Store at -20°C |
|---|---|
| 溶解度 | DMSO：5mg/mL；甲醇：5mg/mL |
| 形态 | 灰白色至浅棕色固体。 |
| 颜色 | 粘黄色 |
Bafilomycin C1 是从链霉菌中分离得到的大环内酯类抗生素 (antibiotic)。Bafilomycin C1 是一种有效的、特异性的、可逆的 vacuolar-type H+-ATPases 抑制剂。Bafilomycin C1 抑制革兰氏阳性菌 (bacteria) 和真菌 (fungi) 的生长。Bafilomycin C1 可诱导细胞凋亡 (apoptosis)，可用于肝细胞癌 (HCC) 的研究。
Bafilomycin C1 (0.33-10 μM; 6 days) inhibits the growth and proliferation of SMMC7721 and HepG2 cells in a timeand dose-dependent manner.Bafilomycin C1 (0.33-3.3 μM; 24 hours) decreases cyclin D3, cyclin E1, CDK2, CDK4, and CDK6 expression in both mRNA and protein expression in SMMC7721 cells.Bafilomycin C1 (3.3-10 μM; 24 hours) causes morphological alterations and increases the population of apoptotic cells by Hoechst 33258 (HY-15558) staining compared to vehicle.
Cell Viability Assay
| Cell Line: | SMMC7721 cell and HepG2 cell |
| Concentration: | 0.33 μM, 1.1 μM, and 3.3 μM for SMMC77211.1 μM, 3.3 μM, and 10.0 μM for HepG2 |
| Incubation Time: | 6 days |
| Result: | Retarded the cell growth. |
Western Blot Analysis
| Cell Line: | SMMC7721 cells |
| Concentration: | 3.3 μM |
| Incubation Time: | 24 hours |
| Result: | Decreaed cyclin D3/E1,CDK2/4/6 protein expression and increased p21. |
Apoptosis Analysis
| Cell Line: | SMMC7721 and HepG2 cells |
| Concentration: | 3.3 μM; 10 μM |
| Incubation Time: | 24 hours |
| Result: | Induced apoptosis in SMMC7721 and HepG2 cells. |
Bafilomycin C1 (subcutaneous injection; 0.2 mg/kg; 20 days) retards the tumor growth without apparent adverse reactions or side effects in nude mice model.
| Animal Model: | BALB/c nude mice (weighing 18-20 g) subcutaneous injected by SMMC7721 cell suspension (5×10 6 cells/100 μL) |
| Dosage: | 0.2 mg/kg |
| Administration: | Subcutaneous injection; 20 days |
| Result: | Suppressed tumor growth of SMMC7721 tumor xenografts. |
安全信息
| 更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
|---|---|---|---|---|---|
| 2024/11/08 | HY-130173 | 巴佛洛霉素C 1 Bafilomycin C1 | 88979-61-7 | 1mg | 1980元 |
| 2024/04/30 | HY-130173 | 巴佛洛霉素C 1 Bafilomycin C1 | 88979-61-7 | 5 mg | 9800元 |