Histatin 5 (TFA)
中文名称 | Histatin 5 (TFA) |
---|---|
中文同义词 | 富组蛋白5三氟乙酸盐 |
英文名称 | Histatin 5 (TFA) |
英文同义词 | Histatin 5 (TFA) |
CAS号 | |
分子式 | |
分子量 | 0 |
EINECS号 | |
相关类别 | 目录多肽 |
Mol文件 | Mol File |
结构式 |
Histatin 5 (TFA) 性质
形态 | 固体 |
---|---|
颜色 | 白色至米白色 |
IC 50 : 0.57 μM (MMP-2), 0.25 μM (MMP-9)
Histatin 5 is a member of a family of low-molecular-weight salivary proteins secreted by parotid, submandibular, and sublingual glands. Using biotinylated gelatin as a substrate, Histatin 5 is found to inhibit the activity of the host matrix metalloproteinases MMP-2 and MMP-9 with IC 50s of 0.57 and 0.25 μM, respectively. To localize the domain responsible for this inhibition, three peptides containing different regions of Histatin 5 are synthesized and tested as inhibitors of MMP-9. Peptides comprising residues 1 to 14 and residues 4 to 15 of Histatin 5 show much lower inhibitory activities (IC 50s , 21.4 and 20.5 μM, respectively), while a peptide comprising residues 9 to 22 showed identical activity to Histatin 5 against MMP-9. Kinetic analysis of the inhibition of the Arg-gingipain reveals that Histatin 5 is a competitive inhibitor, affecting only the Km with a Ki of 15 μM . Histatin 5 is an inhibitor of mitochondrial respiration.The human salivary antifungal peptide Histatin 5 is taken up by Candida albicans cells and associates intracellularly with mitochondria. Histatin 5 inhibits respiration of isolated C. albicans mitochondria as well as the respiration of intact blastoconidia in a dose and time-dependent manner. Histatin 5 at a concentration of 33 uM inhibits state 2 respiration.