基本信息
| 中文名称 | EthidiuM-d5 BroMide |
|---|---|
| 中文同义词 | |
| 英文名称 | EthidiuM-d5 BroMide |
| 英文同义词 | EthidiuM-d5 BroMide;2,7-DiaMino-10- (ethyl-d5)-9-phenylphenanthridiniuM BroMide;2,7-DiaMino-9-phenyl-10-(ethyl-d5) phenanthridiniuM BroMide;2,7-DiaMino-9-phenylphenanthridine-d5 EthobroMide;3,8-DiaMino-5-(ethyl-d5)-6-phenylphenanthridiniuM BroMide;DroMilac-d5;HoMidiuM-d5 BroMide |
| CAS号 | |
| 分子式 | C21H20BrN3 |
| 分子量 | 394.3076 |
| EINECS号 | |
| 相关类别 | Amines;Aromatics;Intermediates & Fine Chemicals;Isotope Labelled Compounds;Pharmaceuticals |
| Mol文件 | Mol File |
| 结构式 |  |
EthidiuM-d5 BroMide 性质
用途
Labelled Ethidium bromide (EtBr) (E890700). It is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-s tranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluo rimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of D NA polymerase. Antiprotozoal (Trypanosoma).