| Company Name: |
Sigma-Aldrich
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| Tel: |
021-61415566 800-8193336 |
| Email: |
orderCN@merckgroup.com |
| Products Intro: |
Product Name:ExtrAvidinPeroxidase
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| Company Name: |
Riedel-de Haen AG
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| Tel: |
800 558-9160 |
| Email: |
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| Products Intro: |
Product Name:ExtrAvidinR-Phycoerythrin
Purity:buffered aqueous solution
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| Company Name: |
SIGMA-RBI
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| Tel: |
800 736 3690 (Orders) |
| Email: |
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| Products Intro: |
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| | EXTRAVIDIN Basic information |
| | EXTRAVIDIN Chemical Properties |
| storage temp. | -20°C | | form | essentially salt-free, lyophilized powder |
| Hazard Codes | Xn | | Risk Statements | 21/22 | | Safety Statements | 36/37 | | WGK Germany | 3 |
| | EXTRAVIDIN Usage And Synthesis |
| Uses | ExtrAvidin?-Peroxidase is suitable for the following applications:
- Dot blot at a minimum dilution of 1:4,000
- Immunohistochemistry (using formalin-fixed, paraffin-embedded sections at a dilution of 1:100)
- Direct ELISA at a minimum dilution of 1:2,000
It has also been used for:
- Immunoprecipitation and Immunobloting
- Ra2I solid-phase binding assays
- Southwestern blotting
- Western blotting
- Immunocytochemistry
| | Uses | ExtrAvidin?-Cy3? is suitable for the following applications:
- Immunohistochemistry (formalin-fixed, paraffin-embedded sections) at a dilution of 1:100
- Immunocytochemistry.
- Used for labeling.
| | General Description | Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin is prepared from egg white avidin. It is a modified form of affinity purified avidin and combines high specific activity of avidin with the low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin has been conjugated to fluorescein isothiocyanate (FITC) and is used for various techniques. | | Biochem/physiol Actions | ExtrAvidin? is prepared from egg white avidin. It is a tetrameric protein containing four high affinity binding sites for biotin. It combines the high specific activity of avidin with the low background staining of streptavidin, a biotin binding protein produced by the bacteria Streptomyces avidinii. It binds biotin with the high affinity of egg white avidin, however, it does not exhibit the unwanted non-specific binding reported for egg white avidin at physiological pH, such as the staining of mast cells. |
| | EXTRAVIDIN Preparation Products And Raw materials |
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