组胺素 5
| 中文名称 | 组胺素 5 |
|---|---|
| 中文同义词 | 组胺素 5;富组蛋白5;组氨素5;抑制剂多肽HISTATIN 5 (TFA) |
| 英文名称 | Histatin 5 |
| 英文同义词 | Histatin 5 (synthetic human re-Hst5);Human histatin 5;Peptide F-A (human parotid saliva);L-Tyrosine, L-α-aspartyl-L-seryl-L-histidyl-L-alanyl-L-lysyl-L-arginyl-L-histidyl-L-histidylglycyl-L-tyrosyl-L-lysyl-L-arginyl-L-lysyl-L-phenylalanyl-L-histidyl-L-α-glutamyl-L-lysyl-L-histidyl-L-histidyl-L-seryl-L-histidyl-L-arginylglycyl- |
| CAS号 | 115966-68-2 |
| 分子式 | C133H195N51O33 |
| 分子量 | 3036.29 |
| EINECS号 | |
| 相关类别 | 标准品 |
| Mol文件 | 115966-68-2.mol |
| 结构式 |  |
组胺素 5 性质
| 密度 | 1.57±0.1 g/cm3(Predicted) |
|---|---|
| 储存条件 | Store at -20°C |
| 溶解度 | 溶于二甲基亚砜 |
| 形态 | 固体 |
IC50: 0.57 μM (MMP-2), 0.25 μM (MMP-9)
Histatin 5 is a member of a family of low-molecular-weight salivary proteins secreted by parotid, submandibular, and sublingual glands. Using biotinylated gelatin as a substrate, Histatin 5 inhibits the activity of the host matrix metalloproteinases MMP-2 and MMP-9 with IC 50 s of 0.57 and 0.25 μM, respectively. To localize the domain responsible for this inhibition, three peptides containing different regions of Histatin 5 are synthesized and tested as inhibitors of MMP-9. Peptides comprising residues 1 to 14 and residues 4 to 15 of Histatin 5 show much lower inhibitory activities (IC 50 , 21.4 and 20.5 μM, respectively), while a peptide comprising residues 9 to 22 showed identical activity to Histatin 5 against MMP-9. Kinetic analysis of the inhibition of the Arg-gingipain reveals that Histatin 5 is a competitive inhibitor, affecting only the K m with a K i of 15 μM. Histatin 5 is an inhibitor of mitochondrial respiration.The human salivary antifungal peptide Histatin 5 is taken up by Candida albicans cells and associates intracellularly with mitochondria. Histatin 5 inhibits respiration of isolated C. albicans mitochondria as well as the respiration of intact blastoconidia in a dose and time-dependent manner. Histatin 5 at a concentration of 33 μM inhibits state 2 respiration.