| Company Name: |
Sigma-Aldrich
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| Tel: |
021-61415566 800-8193336 |
| Email: |
orderCN@merckgroup.com |
| Products Intro: |
Product Name:PCR DIG Labeling Mix
Purity:PCR DIG Labeling MixPLUS Package:1EA Remarks:11585550910
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| | PCR DIG LABELING MIX Basic information |
| Product Name: | PCR DIG LABELING MIX | | Synonyms: | PCR DIG LABELING MIX;PCR DIG LABELING MIXPLUS | | CAS: | | | MF: | | | MW: | 0 | | EINECS: | | | Product Categories: | | | Mol File: | Mol File | ![PCR DIG LABELING MIX Structure]() |
| | PCR DIG LABELING MIX Chemical Properties |
| storage temp. | -20°C | | form | solution |
| | PCR DIG LABELING MIX Usage And Synthesis |
| Uses | PCR DIG Labeling MixPLUS is used for direct labeling of amplification products with digoxigenin (DIG)-deoxyuridine triphosphate (dUTP) in polymerase chain reactions (PCR) and for carryover prevention.The PCR DIG Labeling Mixplus is 10x concentrated. For each reaction, add 10% of the final volume to the reaction mix. | | General Description | The PCR DIG Labeling MixPLUS is a mixture of lithium salts of deoxyadenosine triphosphate (dATP), deoxycytosine triphosphate (dCTP), deoxyguanosine triphosphate (dGTP), deoxyuridine triphosphate (dUTP) and digoxigenin (DIG)-11-dUTP. This nucleotide mixture can be added directly to polymerase chain reactions (PCR) and the DIG-labeled nucleotide will be incorporated into the PCR product. The incorporation of dUTP instead of deoxythymidine triphosphate (dTTP) allows the degradation of contaminating PCR products from previous amplification reactions with uracil-DNA glycosylase (UNG), preventing carryover contamination.The incorporated DIG label renders the sensitive detection of the PCR product with anti-DIG-conjugates, for example in the PCR ELISA, DIG-labling, and PCR ELISA, DIG-Detection. The PCR DIG Labeling MixPLUS gives comparable signal intensities to the PCR DIG Labeling Mix. |
| | PCR DIG LABELING MIX Preparation Products And Raw materials |
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