| Company Name: |
Riedel-de Haen AG
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| Tel: |
800 558-9160 |
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| Products Intro: |
Remarks:affinity isolated antibody, buffered aqueous solution
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| Company Name: |
SIGMA-RBI
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| Tel: |
800 736 3690 (Orders) |
| Email: |
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| Products Intro: |
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| | ANTI-GUANYLATE CYCLASE B1 (ER-19) RABBIT Basic information |
| | ANTI-GUANYLATE CYCLASE B1 (ER-19) RABBIT Chemical Properties |
| storage temp. | -20°C | | form | buffered aqueous solution |
| | ANTI-GUANYLATE CYCLASE B1 (ER-19) RABBIT Usage And Synthesis |
| Uses | Anti-Guanylyl Cyclase β1 (ER-19) antibody produced in rabbit is suitable for immunoblotting at a working dilution of 1:2000 using a cytosolic fraction of rat brain, immunoprecipitation at a working antibody amount of 2-3μg using 60-120μg of a cytosolic fraction of rat brain and for immunohistochemistry at 1:400 using trypsin-digested human, bovine and mouse heart tissue.
It has been used as a primary antibody for:
- localization of β1 subunits of sGC (soluble guanylate cyclase) in the guinea pig gastrointestinal tract
- detection of expression of sGC in the vasculature of rat skeletal muscle
- localization of the functional subunit of NO receptors, sGCβ1 in guinea pig caecum
It has also been used in immunoblot assay to determine whether there were changes in uterine vascular smooth muscle (UVSM) sGC expression during the ovine reproductive cycle. | | General Description | Soluble guanylyl cyclase (sGC) is an obligate hemoprotein enzyme consisting of α and β subunits of ~80 kDa and ~70 kDa, respectively. | | Biochem/physiol Actions | The enzyme (GC) is a major physiological receptor for nitric oxide (NO), an important intra- and intercellular membrane-permeant signaling molecule. Gaseous NO binds to Fe2+ in the prosthetic heme group of the enzyme. NO binding is followed by disruption of the β1 subunit histidine105 bond to iron and activation of the enzyme. GC forms a complex with NO and cGMP and regulates smooth muscle relaxation, inflammation, platelet adhesion and aggregation, pulmonary physiology and neuronal function. It is an important target for NO-releasing and non-NO-releasing activator drugs in human cardiovascular therapy. |
| | ANTI-GUANYLATE CYCLASE B1 (ER-19) RABBIT Preparation Products And Raw materials |
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