- Luciferase, firefly
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- $1220.00 / 10mg
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2024-10-28
- CAS:61970-00-1
- Min. Order:
- Purity:
- Supply Ability: 10g
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| | LUCIFERASE Basic information |
| | LUCIFERASE Chemical Properties |
| Fp | 111℃ | | storage temp. | -20°C | | form | powder | | color | white | | Specific Activity | 15-30×106light units/mg protein |
| | LUCIFERASE Usage And Synthesis |
| Description | Luciferase (firefly recombinant) is a light-generating enzyme that catalyzes an oxidative reaction, using luciferin, ATP, and molecular oxygen to produce oxyluciferin, which yields visible light. The bioluminescence of this ATP-dependent luciferase is commonly used in reporter systems that are amenable to high-throughput screening. | | Uses | Firefly luciferase is used extensively in molecular and cell biology, in particular for the efficient detection and quantitation of ATP and as a reporter for genetic function. | | Uses | Luciferase from Photinus pyralis (firefly) has been used as a component of lysis solution to measure luminescence signals, as part of the study to determine chemical signals which can activate the karrikin insensitive 2 (KAI2) pathway. | | General Description | Firefly luciferase catalyzes the reaction of luciferin with ATP and leads to the production of yellow-green light. The enzyme has a molecular weight of 62kDa and is expressed in peroxisomes. Luciferase is considered as a model to study protein–anesthetic interactions. Firefly luciferase is highly useful in cell biology and molecular biology, as a reporter of gene function and for the quantification of ATP. | | Biochem/physiol Actions | Firefly luciferase is a 62 kDa protein that catalyzes the production of light. The enzyme requires ATP, molecular oxygen, and the heterocyclic compound, firefly luciferin, to generate light in a two-step process. The light producing reaction is initiated by luciferin activation (adenylation of its carboxylate group) and proceeds in the presence of molecular oxygen to yield a photon of yellow-green light. |
| | LUCIFERASE Preparation Products And Raw materials |
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