| Company Name: |
Sigma-Aldrich
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| Tel: |
021-61415566 800-8193336 |
| Email: |
orderCN@merckgroup.com |
| Products Intro: |
Product Name:RPMI-1640 Medium
Purity:Modified, with 20 mM HEPES and L-glutamine, without sodium b Package:6X500ML Remarks:R7388-6X500ML
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| | RPMI 1640 MEDIUM Basic information |
| Product Name: | RPMI 1640 MEDIUM | | Synonyms: | MEGACELL RPMI-1640 MEDIUM;CLICK RPMI-MEDIUM;CELLECT(TM) DEFICIENT MEDIUM WITHOUT L-LEUCINE;CELLECT(TM) DEFICIENT MEDIUM WITHOUT L-METHIONINE, L-CYSTEINE AND L-CYSTINE;CELLECT(TM) DEFICIENT MEDIUM WITHOUT PHENOL RED;CELLECT(TM) DEFICIENT MEDIUM WITHOUT L-METHIONINE;CELLECT(TM) DEFICIENT MEDIUM WITHOUT PHOSPHATE OR GLUCOSE;DME X | | CAS: | | | MF: | | | MW: | 0 | | EINECS: | | | Product Categories: | Cell Culture;Classic Media and Salts;MegaCellTM Media | | Mol File: | Mol File | ![RPMI 1640 MEDIUM Structure]() |
| | RPMI 1640 MEDIUM Chemical Properties |
| storage temp. | 2-8°C | | form | powder | | PH Range | >7.2 |
| | RPMI 1640 MEDIUM Usage And Synthesis |
| Uses | RPMI-1640 Medium is used for the following applications:
- Used in cell culture medium: Murine thymoma EL-4 cells were cultured in RPMI 1640 medium supplemented with other components
- Used for the mycobacterial infection of splenocytes (in antibiotic-free RPMI 1640 medium)
- Used for cell isolation and culture
- Used in culture medium (as one of the component) during lymphocyte separation and culture, ELISPOT Assay, Ex Vivo Proliferation Assay
- Used in medium for gp39 gene expression and CD40-Immunoglobuin binding assays
- Used for the preparation of antifungal agents
| | Uses | RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. | | General Description | RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. |
| | RPMI 1640 MEDIUM Preparation Products And Raw materials |
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