化合物 T10325
中文名称 | 化合物 T10325 |
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中文同义词 | 化合物 T10325;化合物ANI-7;(Z)-2-(3,4-二氯苯基)-3-(1H-吡咯-2-基)丙烯腈 |
英文名称 | ANI-7 |
英文同义词 | ANI-7;ANI 7,ANI7;Benzeneacetonitrile, 3,4-dichloro-α-(1H-pyrrol-2-ylmethylene)-, (αZ)-;(Z)-2-(3,4-Dichlorophenyl)-3-(1H-pyrrol-2-yl)acrylonitrile , ANI-7;(Z)-2-(3,4-Dichlorophenyl)-3-(1H-pyrrol-2-yl)acrylonitrile |
CAS号 | 931417-26-4 |
分子式 | C13H8Cl2N2 |
分子量 | 263.12 |
EINECS号 | |
相关类别 | 对照品 |
Mol文件 | 931417-26-4.mol |
结构式 |
化合物 T10325 性质
沸点 | 443.0±45.0 °C(Predicted) |
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密度 | 1.398±0.06 g/cm3(Predicted) |
储存条件 | Store at -20°C |
溶解度 | 二甲基亚砜:20.83 mg/mL(79.17 mM) |
酸度系数(pKa) | 15.79±0.50(Predicted) |
形态 | 固体 |
颜色 | 浅黄至黄色 |
Aryl Hydrocarbon Receptor
|
Chk2
|
ANI-7 (2.5 μM; 24 hours; MCF10A and MDA-MB-468 cells) treatment induces significant S-phase and G2 + M-phase cell cycle arrest within 24 hours of treatment in MDA-MB-468 cells, and negligible effect in normal breast MCF10A cells.
ANI-7 (2 μM; 12-24 hours; MDA-MB-468 cells) treatment results in a significant increase in the content and phosphorylation of CHK2, and induces a significant increase in H2AXɣ in MDA-MB-468 cells, indicative of DNA double-strand damage.
Inhibition of the AhR pathway ameliorates the effects of ANI-7. ANI-7 activates XRE activity and expression of the AhR and CYP1 members.
Comparisons of the GI
50
values show that ANI-7 produces a GI
50
value of 0.38 μM in MCF-7 cells, whereas values of 3.0-42 μM are observed in cell lines from lung, colon, ovary, neuronal, glial, prostate, and pancreas. The only other tumor type that shows appreciable growth inhibition by ANI-7 is the A431 vulva cell line (GI
50
of 0.51μM).
ANI-7 potently inhibits the growth of T47D, ZR-75-1, MCF-7, SKBR3, and MDA-MB-468 breast cancer cells (GI
50
range of 0.16-0.38 μM), moderately inhibits the growth of BT20 and BT474 cells (GI50 range of 1-2 μM), and essentially fails to inhibit the growth of MDA-MB-231 and MCF10A cells (GI
50
range of 17-26 μM). Moreover, ANI-7 maintained its ability to inhibit the growth of drug-resistant cells (MCF-7/VP16: GI
50
of 0.21 μM).
Cell Cycle Analysis
Cell Line: | MCF10A and MDA-MB-468 cells |
Concentration: | 2.5 μM |
Incubation Time: | 24 hours |
Result: | Induced significant S-phase and G2 + M-phase cell cycle arrest in MDA-MB-468 cells, and negligible effect in normal breast MCF10A cells. |
Western Blot Analysis
Cell Line: | MDA-MB-468 cells |
Concentration: | 2 μM |
Incubation Time: | 12 hours, 24 hours |
Result: | Resulted in a significant increase in the content and phosphorylation of CHK2 (25-fold increase),and induced a significant increase in H2AXɣ (3.5-fold increase). |
安全信息
更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
---|---|---|---|---|---|
2024/08/19 | HY-117102 | 化合物 T10325 ANI-7 | 931417-26-4 | 5mg | 700元 |
2024/08/19 | HY-117102 | 化合物 T10325 ANI-7 | 931417-26-4 | 10mM * 1mLin DMSO | 770元 |