CITCO
| 中文名称 | CITCO |
|---|---|
| 中文同义词 | 6-(4-氯苯基)咪唑并[2,1-B]噻唑-5-甲醛 O-(3,4-二氯苄基)肟;化合物CITCO |
| 英文名称 | CITCO |
| 英文同义词 | Imidazo[2,1-b]thiazole-5-carboxaldehyde, 6-(4-chlorophenyl)-, O-[(3,4-dichlorophenyl)methyl]oxime;6-(4-Chlorophenyl)imidazo[2,1-b]thiazole-5-carbaldehyde O-(3,4-dichlorobenzyl) oxime;CITCO,Inhibitor,inhibit,Apoptosis |
| CAS号 | 338404-52-7 |
| 分子式 | C19H12Cl3N3OS |
| 分子量 | 436.74 |
| EINECS号 | |
| 相关类别 | |
| Mol文件 | 338404-52-7.mol |
| 结构式 |  |
CITCO 性质
| 密度 | 1.48±0.1 g/cm3(Predicted) |
|---|---|
| 储存条件 | -20°C |
| 溶解度 | DMSO：28 mg/mL，可溶 |
| 形态 | 固体 |
| 酸度系数(pKa) | 4.05±0.40(Predicted) |
| 颜色 | 浅黄至黄色 |
CAR, PXR
CITCO (1-50 μM; 48 hours) results in a dose-dependent inhibition of viable cell count and proliferation in both T98G and U87MG glioma and BTSCs.
CITCO (2.5, 5 μM; 48 hours) induces cell cycle arrest differentially in different BTSCs in culture, but not in normal astrocytes.
CITCO (2.5-10 μM; 48 hours) induces apoptosis in BTSCs in culture in dose dependently, but not in normal astrocytes.
CITCO (0-25 μM; 48 hours) causes the T98G and U87MG glioma and BTSCs expressing very low levels of CAR protein that increased significantly.
Cell Proliferation Assay
| Cell Line: | T98G, U87MG, DB29 and DB33 human glioma cells, astrocytes |
| Concentration: | 1, 2.5, 5, 10, 25, 50 μM |
| Incubation Time: | 48 hours |
| Result: | Resulted in a dose-dependent inhibition of viable cell count and proliferation. |
Cell Cycle Analysis
| Cell Line: | The T98G, U87MG, DB29 and DB33 glioma cells |
| Concentration: | 2.5, 5 μM |
| Incubation Time: | 48 hours |
| Result: | Induced cell cycle arrest differentially in different BTSCs in culture. |
Apoptosis Analysis
| Cell Line: | The T98G, U87MG, DB29 and DB33 glioma cells |
| Concentration: | 2.5, 5 or 10 μM |
| Incubation Time: | 48 hours |
| Result: | Increased the levels of Annexin V-positive apoptotic cells in dose dependently. |
Western Blot Analysis
| Cell Line: | T98G, U87MG, DB29 and DB33 glioma cells |
| Concentration: | 0 to 25 μM |
| Incubation Time: | 48 hours |
| Result: | The T98G, U87MG glioma and BTSCs expressed very low levels of CAR protein that increased significantly. |
CITCO (intraperitoneal; on days 22, 24, 26, 30 and 36) with 25 μg results a significant decrease in tumour growth, which further decreases to an undetectable level after treatment with 100 μg CITCO .
| Animal Model: | Six- to eight-week-old male athymic nude mice |
| Dosage: | 25 or 100 μg |
| Administration: | Intraperitoneal; on days 22, 24, 26, 30 and 36 |
| Result: | Decreased tumour growth. |