BOHEMINE
| 中文名称 | BOHEMINE |
|---|---|
| 中文同义词 | 3-{[6-(苄基氨基)-9-异丙基-9H-嘌呤-2-基]氨基}-1-丙醇;3-((6-(苄基氨基)-9-异丙基-9H-嘌呤-2-基)氨基)丙-1-醇;化合物BOHEMINE |
| 英文名称 | BOHEMINE |
| 英文同义词 | 3-((6-(benzylaMino)-9-isopropyl-9H-purin-2-yl)aMino)propan-1-ol;3-{[6-(Benzylamino)-9-Isopropyl-9H-Purin-2-Yl]Amino}-1-Propanol;1-Propanol,3-[[9-(1-methylethyl)-6-[(phenylmethyl)amino]-9H-purin-2-yl]amino]-;CDK,MAb,inhibit,rearrangement,Bohemine,proteosynthesis,Extracellular signal regulated kinases,Inhibitor,Cyclin dependent kinase,dk9/cyclin T1,ERK,cytoskeleton,Cdk2/cyclin A,purine,anti-cancer,Cdk2/cyclin E |
| CAS号 | 189232-42-6 |
| 分子式 | C18H24N6O |
| 分子量 | 340.42 |
| EINECS号 | |
| 相关类别 | 小分子抑制剂;小分子抑制剂，天然产物;Inhibitors |
| Mol文件 | 189232-42-6.mol |
| 结构式 |  |
BOHEMINE 性质
| 熔点 | 101-103 °C |
|---|---|
| 沸点 | 589.4±60.0 °C(Predicted) |
| 密度 | 1.27±0.1 g/cm3(Predicted) |
| 储存条件 | Keep in dark place,Sealed in dry,2-8°C |
| 溶解度 | 氯仿：可溶50 mg/mL |
| 形态 | 粉末 |
| 酸度系数(pKa) | 14.94±0.10(Predicted) |
| 颜色 | 白色至米白色 |
| Target | Value |
|
Cdk9/cyclin T1
() | 2.7 μM |
|
Cdk2/cyclin E
() | 4.6 μM |
|
Cdk2/cyclin A
() | 83 μM |
Bohemine (0-30 µM; 72 hours; ME-750 cells) treatment inhibits cell growth. Addition of Bohemine at concentrations in the range of 1-10 µM results in a short-term arrest of growth and of monoclonal antibody production. The short-term suppression of cell functions is followed by a significant temporary increase of specific growth rate and of specific production rate.
Hybridoma cells are retarded both at the G1/S boundary and at the G2/M boundary, depending on Bohemine (0-30 µM) concentration.
T-lymphoblastic cell line CEM is treated by Bohemine, five proteins are found to be downregulated, namely α-enolase, triosephosphate isomerase, initiation factor 5A, and α- and β-subunits of Rho GDP-dissociation inhibitor 1. These proteins play significant roles in glycolysis, proteosynthesis, and in cytoskeleton rearrangement.
Bohemine inhibits growth of human tumor cell lines with an IC
50
of 27 µM.
Cell Viability Assay
| Cell Line: | Mouse hybridoma ME-750 cells |
| Concentration: | 0 µM, 1 µM, 3 µM, 10 µM and 30 µM |
| Incubation Time: | 72 hours |
| Result: | At 10 µM and 30 µM concentrations, the viable cell count was significantly lower with respect to control, i.e., 77% and 48%, respectively. |
Bohemine (50 mg/kg; intravenous injection; BALB/c mice) treatment shows C max is 72,308 nM, observed clearance is 0.23 L/h and T 1/2 is 1.39 h.
| Animal Model: | BALB/c mice bearing the colon 26 murine tumor |
| Dosage: | 50 mg/kg |
| Administration: | Intravenous injection (Pharmacokinetic Analysis) |
| Result: | C max is 72,308 nM, observed clearance is 0.23 L/h and T 1/2 is 1.39 h. |
安全信息
| WGK Germany | 3 |
|---|
| 更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
|---|---|---|---|---|---|
| 2024/04/30 | S6531 | BOHEMINE Bohemine | 189232-42-6 | 5mg | 794.57元 |
| 2024/04/30 | S6531 | BOHEMINE Bohemine | 189232-42-6 | 25mg | 2432.89元 |