| Company Name: |
Sigma-Aldrich
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| Tel: |
021-61415566 800-8193336 |
| Email: |
orderCN@merckgroup.com |
| Products Intro: |
Product Name:Monoclonal Anti-Fibronectin antibody produced in mouse
Purity:clone IST-3, ascites fluid Package:.2ML Remarks:F0791-.2ML
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| Company Name: |
Riedel-de Haen AG
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| Tel: |
800 558-9160 |
| Email: |
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| Products Intro: |
Product Name:Monoclonal Anti-Fibronectin antibody produced in mouse
Purity:affinity isolated antibody, lyophilized powder Remarks:affinity isolated antibody, buffered aqueous solution
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| Company Name: |
SIGMA-RBI
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| Tel: |
800 736 3690 (Orders) |
| Email: |
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| Products Intro: |
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| Company Name: |
BD Biosciences
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| Tel: |
1 877 232 8995 U.S. orders |
| Email: |
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| Products Intro: |
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| | ANTI-HUMAN FIBRONECTIN Basic information |
| | ANTI-HUMAN FIBRONECTIN Chemical Properties |
| storage temp. | −20°C | | form | buffered aqueous solution |
| Hazard Codes | Xn | | Risk Statements | 21/22 | | Safety Statements | 36/37 | | WGK Germany | 3 |
| | ANTI-HUMAN FIBRONECTIN Usage And Synthesis |
| Uses | Monoclonal Anti-Fibronectin antibody has been used :
- in immunohistochemistry
- in confocal microscopy
- in immunocytochemistry
- western blotting analysis
| | Uses | Mouse monoclonal clone IST-3 anti-Human Fibronectin antibody has been used in:
- enzyme linked immuno sorbent assay (ELISA)
- western blotting
- dot blot
- radio immunoassay (RIA)
- immunocytochemistry
- immunohistochemistry
- immunoprecipitation
- immunofluorescence
| | Uses | Anti-Fibronectin antibody is suitable for
- immunohistochemistry (IHC) analysis to study the activity of pancreatic stellate cells in chronic pancreatitis
- antibody blocking assays of bacterial cells to study the activity of fibronectin in the Yersinia pestis binding to host cells and Yop delivery
- staining of endothelial cells as primary antibody
- cell adhesion assays to study the effect of anti-human fibronectin antibodies on adhesion to endothelial cells
- immunoprecipitation and western blot analysis of NF-κB dependent mammary tumorigenesis study
- immunofluorescence staining to study the correlation between zyxin tails and fibrillar adhesions
- immunoblotting to study the expression levels of the pancreatic stellate cells (PSC) markers glial fibrillary acidic protein (GFAP), α-smooth muscle actin (α-SMA), collagen type I, and fibronectin in whole cell lysates
- ELISA of fibronectin secretion
- microarray analysis.
| | General Description | Monoclonal Anti-Fibronectin (mouse IgG1 isotype) is derived from the hybridoma produced the fusion of mouse myeloma cells and splenocytes from immunized mice. Fibronectin (FN) belongs to glycoprotein family widely expressed in many tissues. FN is an extracellular matrix protein composed of two nearly-identical disulfide-bound polypeptides with typical molecular weights of 220-240 kDa. There are three isoforms of fibronectin, that includes cellular FN, plasma FN and fetal FN. Cellular fibronectin is structurally and antigenically similar to cold insoluble globulin from plasma and antibodies to either form usually cross-react. Careful analysis of the FN molecule indicates that it contains several functionally and structurally distinct domains which may bind to cell surfaces and to a variety of molecules such as collagen, heparin, gelatin, fibrin and DNA. FN gene in human chromosome is mapped to 2q35. | | Biochem/physiol Actions | Fibronectin (FN) play an important role in diverse biological phenomena including cell adhesion, cell migration, hemostasis and thrombosis, wound healing and the ability to induce a more normal phenotype in transformed cells. FN is upregulated in many cancers. FN increases the expression of matrix metalloproteinases (MMPs), promoting cancer cell invasion and metastasis. |
| | ANTI-HUMAN FIBRONECTIN Preparation Products And Raw materials |
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