| Company Name: |
Beijing Ouhe Technology Co., Ltd
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| Tel: |
010-82967028 13552068683 |
| Email: |
2355560935@qq.com |
| Products Intro: |
Product Name:Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)*
CAS:136632-31-0
Purity:98% Package:25 nmoles
|
| Company Name: |
Sigma-Aldrich
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| Tel: |
021-61415566 800-8193336 |
| Email: |
orderCN@merckgroup.com |
| Products Intro: |
Product Name:Biotin-16-dUTP
CAS:136632-31-0
Purity:Biotin-16-dUTP Package:1EA Remarks:11093070910
|
| Company Name: |
Energy Chemical
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| Tel: |
021-58432009 400-005-6266 |
| Email: |
marketing@energy-chemical.com |
| Products Intro: |
Product Name:Biotin-16-dUTP
CAS:136632-31-0
Purity:NULL Package:1EA Remarks:NULL
|
| Company Name: |
amyjetsci
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| Tel: |
027-59626688 18771149750 |
| Email: |
sales@amyjet.com |
| Products Intro: |
Product Name:Biotin-16-dUTP.1 mM in Tris Buffer(pH 7.5).
Package:25nmoles/
|
| Company Name: |
Hubei Aiputi Bioengineering Co., Ltd.
|
| Tel: |
17762444226 |
| Email: |
d17762444226@163.com |
| Products Intro: |
Product Name:Biotin-16-dUTP.1 mM in Tris Buffer(pH 7.5).
CAS:136632-31-0
Package:750nmole
|
|
| | Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)* Basic information |
| | Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)* Chemical Properties |
| storage temp. | -20°C | | form | solution |
| | Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)* Usage And Synthesis |
| Uses | For nonradioactive DNA labeling such as random priming, PCR labeling, or nick translation.
- Biotin-16-dUTP is used as asubstrate for: Terminal Transferase
- DNA polymerase I (holoenzyme and Klenow fragment)
- Taq DNA polymerase
- Reverse transcriptase (e.g., Transcriptor)
Biotin-16-dUTP replaces dTTP in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% Biotin-16-dUTP and 65% dTTP, as well as in PCR.
The nucleotide also serves as a substrate for Terminal Transferase in 3′-end labeling.
- Biotin-labeled DNA can be detected with: Streptavidin-alkaline phosphatase conjugate and a chemiluminescent substrate (CSPD, CDP-Star) or a color substrate
- Biotin Luminescence Detection Kit
| | General Description | Biotin is a water-soluble vitamin which functions as a coenzyme for carboxylases. | | Biochem/physiol Actions | Biotin influences gene regulation and chromatin structure by associating with lysine residues in histones. It influences the actions of adaptive immune T and natural killer cells. Deficiency of biotin in mice has indicated enhanced inflammation. Studies have also indicated heightened inflammatory response of human dendritic cells in conditions of biotin deficiency. |
| | Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)* Preparation Products And Raw materials |
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