| Company Name: |
Sigma-Aldrich
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| Tel: |
021-61415566 800-8193336 |
| Email: |
orderCN@merckgroup.com |
| Products Intro: |
Product Name:Anti-Human IgG (Fab specific)Peroxidase
Purity:affinity isolated antibody Package:1mL Remarks:A0293
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| Company Name: |
Riedel-de Haen AG
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| Tel: |
800 558-9160 |
| Email: |
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| Products Intro: |
Purity:affinity isolated antibody
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| Company Name: |
SIGMA-RBI
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| Tel: |
800 736 3690 (Orders) |
| Email: |
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| Products Intro: |
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| | ANTI-IMMUNOGLOBULIN G, HUMAN, F(AB')2, HRP Basic information |
| Product Name: | ANTI-IMMUNOGLOBULIN G, HUMAN, F(AB')2, HRP | | Synonyms: | F(AB')2 ANTI-HUMAN IGG F(AB')2, HORSERADISH PEROXIDASE;F(AB')2 OF ANTI-HUMAN IGG F(AB')2, HORSERADISH PEROXIDASE;GOAT ANTI-HUMAN IGG F(AB')2, PEROXIDASE CONJUGATED;GOAT ANTI-HUMAN-IGG (H+L) F(AB') PEROXIDASE;ANTI-IGG, F(AB')2 HORSERADISH PEROXIDASE CONJUGATED;ANTI-IGG F(AB')2 FRAGMENT, HUMAN PEROXIDASE CONJ;ANTI-IMMUNOGLOBULIN G, HUMAN, F(AB')2, HRP;ANTI-HUMAN IGG F(AB')2, HORSERADISH PEROXIDASE | | CAS: | | | MF: | | | MW: | 0 | | EINECS: | | | Product Categories: | Alphabetical Index;Antibodies;by Animal;by Label;HSecondary Antibodies;Human IgG Secondary Antibodies and ConjugatesSecondary Antibodies;Peroxidase | | Mol File: | Mol File | ![ANTI-IMMUNOGLOBULIN G, HUMAN, F(AB')2, HRP Structure]() |
| | ANTI-IMMUNOGLOBULIN G, HUMAN, F(AB')2, HRP Chemical Properties |
| storage temp. | −20°C | | form | buffered aqueous solution |
| | ANTI-IMMUNOGLOBULIN G, HUMAN, F(AB')2, HRP Usage And Synthesis |
| Uses | Anti-Human IgG (Fab specific)-Peroxidase antibody produced in goat has been used in:
- western blot
- direct enzyme linked immunosorbent assay (ELISA) at 1:40,000 dilution for analysis of yeast cultures genetically modified to produced recombinant Fab fragments.
- immunohistochemistry
- dot blot
- immunohistochemistry (formalin-fixed, paraffin-embedded sections, 1:200)
Anti-Human IgG antibody was used at a 1:10,000 dilution in PBS and incubated for 1 hour at room temperature. Binding in ELISA assays was detected with a O-phenylene-diamine substrate solution (Sigma). | | General Description | Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders,.
Antibody is isolated from anti-human IgG antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fab fragment of human IgG. Anti-Human IgG is conjugated to peroxidase and then further purified to remove unconjugated material. |
| | ANTI-IMMUNOGLOBULIN G, HUMAN, F(AB')2, HRP Preparation Products And Raw materials |
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