Desthiobiotin-PEG4-DBCO manufacturers
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| Desthiobiotin-PEG4-DBCO Basic information |
| Desthiobiotin-PEG4-DBCO Chemical Properties |
Boiling point | 1013.4±65.0 °C(Predicted) | density | 1.24±0.1 g/cm3(Predicted) | pka | 14.93±0.60(Predicted) |
| Desthiobiotin-PEG4-DBCO Usage And Synthesis |
Description | DBCO-PEG4-Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd?= 1011?vs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin. DBCO is commonly used for copper-free Click Chemistry reactions. |
| Desthiobiotin-PEG4-DBCO Preparation Products And Raw materials |
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