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| | Endovion Basic information |
| Product Name: | Endovion | | Synonyms: | Endovion;NS3728;Urea, N-[3,5-bis(trifluoromethyl)phenyl]-N'-[4-bromo-2-(2H-tetrazol-5-yl)phenyl]-;NS3728 >=98% (HPLC);1-(3,5-Bis(trifluoromethyl)phenyl)-3-(4-bromo-2-(2H-tetrazol-5-yl)phenyl)urea;Cl? Channels,Inhibitor,NS 3728,Endovion,Chloride Channel,inhibit,NS-3728 | | CAS: | 265646-85-3 | | MF: | C16H9BrF6N6O | | MW: | 495.18 | | EINECS: | | | Product Categories: | | | Mol File: | 265646-85-3.mol |  |
| | Endovion Chemical Properties |
| density | 1.791±0.06 g/cm3(Predicted) | | storage temp. | Store at -20°C | | solubility | Soluble to 100 mM in DMSO and to 50 mM in ethanol | | pka | 3.95±0.10(Predicted) | | form | Solid | | color | White to off-white | | Water Solubility | Water : < 0.1 mg/mL (insoluble) |
| | Endovion Usage And Synthesis |
| Biological Activity | Endovion (NS3728) is an anion channel inhibitor (eg chloride channel) and a specific VRAC/VSOAC blocker. | | in vitro | Endovion (NS3728, 10-100 μM) reduces TNFα-induced apoptosis and increases p53-protein level as well as downstream signaling, eg, expression of p21 Waf1/Cip1 , Bax, Noxa, MDM2, and activation of Caspase-9/-3 in Cisplatin-sensitive cells.
Endovion (NS3728, 10 μM) inhibits cell proliferation in Capan-1, AsPC-1 and BxPC-3 cell lines.
Western Blot Analysis | Cell Line: | Wild -type, resistant, and transiently transfected A2780 cells. | | Concentration: | 10-100 μM. < /td> | | Incubation Time: | 18 or 4.5 h. | < td class="col1"> Result: Reduced the maximal taurine rate constant more than 90% compared with the untreated control cells.
Resulted i n an increased LRRC8A protein expression.
Significantly reduced p53 and p21Waf1/Cip1 protein level in A2780WT cells.
| Cell Proliferation Assay | Cell Line: | Capan-1, AsPC-1, BxPC-3 and H6c7 cell lines. | | Concentration: | 10 μM. | | Incubation Time: | 24 h. | | Result: | Resulted in the most pronounced inhibition in all cell lines with 77 ± 26 % in Capan-1, 67 ± 9 % in AsPC-1, and 54 ± 8 % in BxPC-3 cells at +67 mV. | | | target | Chloride channel, VRAC/VSOAC.
ANO1. | | storage | Store at -20°C |
| | Endovion Preparation Products And Raw materials |
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