| Company Name: |
Sigma-Aldrich
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| Tel: |
021-61415566 800-8193336 |
| Email: |
orderCN@merckgroup.com |
| Products Intro: |
Product Name:Genopure Plasmid Midi Kit
Purity:Genopure Plasmid Midi Kit Package:1EA Remarks:3143414001
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| | Genopure PlasMid Midi Kit Basic information |
| | Genopure PlasMid Midi Kit Chemical Properties |
| | Genopure PlasMid Midi Kit Usage And Synthesis |
| Uses | The Genopure Plasmid Midi Kit prepares transfection-grade plasmid DNA in medium quantities (up to 100 μg plasmid) from bacterial cultures. Isolated plasmid is suitable for most molecular biology applications:
- Transfection
- Southern blotting
- Sequencing
- PCR
- Restriction analysis
- Cloning
| | General Description | Plasmid recovery was tested with 50 μg purified plasmid. The recovery was >90%, with more than 80% in supercoiled form. The yield of plasmid DNA was determined by isolating pBS from DH5a cells. From 30 mL culture volume with a density of A600 between 3 and 6, >85 μg of plasmid DNA was obtained.
The purity (checked by the ratio of A260/A280) is 1.8 + 0.2.
No RNA is detectable.
The kit components have been tested for the absence of nucleases according to current quality control procedures. |
| | Genopure PlasMid Midi Kit Preparation Products And Raw materials |
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