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| | 5-Ethynyl-2'-deoxycytidine Basic information |
| | 5-Ethynyl-2'-deoxycytidine Chemical Properties |
| Melting point | 191 °C (decomp)(Solv: dichloromethane (75-09-2); methanol (67-56-1)) | | Boiling point | 475.7±55.0 °C(Predicted) | | density | 1.54±0.1 g/cm3(Predicted) | | storage temp. | Store at 2-8°C, protect from light, stored under nitrogen | | solubility | DMF: 5 mg/ml; DMSO: 20 mg/ml; PBS (pH 7.2): 10 mg/ml | | pka | 14.03±0.60(Predicted) | | form | powder to crystal | | color | White to Light yellow to Light orange | | λmax | 292nm(H2O)(lit.) |
| WGK Germany | 3 | | HS Code | 2934.99.4400 |
| | 5-Ethynyl-2'-deoxycytidine Usage And Synthesis |
| Description | 5''-Ethynyl-2''-deoxycytidine (EdC) is a nucleoside analog that inhibits replication of the herpes simplex virus-1 (HSV-1) KOS strain (ID50 = 0.2 μg/mL). It also reduces virus-induced cytopathogenicity of HSV-1, HSV-2, and vaccinia virus strains in PRK cells (MICs = 0.2-0.4, 1-2, and 5 μg/ml, respectively). EdC is an inhibitor of thymidylate synthetase, selectively reducing DNA incorporation of [1'',2''-3H]deoxyuridine over [CH3-3H]deoxythymidine in PRK cells (ID50s = 3 and 120 μg/ml, respectively). It inhibits thymidine synthetase in and reduces proliferation of L1210 cells, an effect which is reversed by addition of deoxythymidine (ID50s = 4.4 and 1,000 μg/ml, respectively). EdC has been used to monitor DNA synthesis and cellular replication via click chemistry conjugation of the ethynyl group to an azido group of various fluorochromes. | | Uses | 5-Ethynyl-2’-deoxycytidine is an lower toxicity analog of 2’-deoxycytidine, a metabolic labeling probe for DNA synthesis. 5-Ethynyl-2’-deoxycytidine is most commonly used when thymidine analogs is undesirable. | | Uses | A relatively nontoxic 2′-deoxycytidine analog for metabolic labeling of newly synthesized DNA in vivo. Especially suitable if the use of thymidine analogs is undesirable. |
| | 5-Ethynyl-2'-deoxycytidine Preparation Products And Raw materials |
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