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| | NAcM-OPT Basic information |
| Product Name: | NAcM-OPT | | Synonyms: | NAcM-OPT; NAcM-OPT NEW;Urea, N-(1-butyl-4-piperidinyl)-N'-(3,4-dichlorophenyl)-N-(phenylmethyl)-;E3 ligating enzyme,Ubiquitin activating enzyme,inhibit,NAcM-OPT,NAE,Ubiquitin conjugating enzyme,NAcMOPT,Ubiquitin ligase,NEDD8-activating Enzyme,E1 activating enzyme,E2 conjugating enzyme,Inhibitor,NAcM OPT,E1/E2/E3 Enzyme | | CAS: | 2089293-61-6 | | MF: | C23H29Cl2N3O | | MW: | 434.4 | | EINECS: | | | Product Categories: | | | Mol File: | 2089293-61-6.mol |  |
| | NAcM-OPT Chemical Properties |
| Boiling point | 589.8±50.0 °C(Predicted) | | density | 1.23±0.1 g/cm3(Predicted) | | storage temp. | -20°C | | solubility | Soluble in DMSO (up to 30 mg/ml) or in Ethanol (up to 20 mg/ml) | | form | solid | | pka | 13.30±0.70(Predicted) | | color | White | | Stability: | Stable for 1 year as from date of purchase supplied. Solutions in DMSO or ethanol may be stored at -20°C for up to 3 months. |
| | NAcM-OPT Usage And Synthesis |
| Description | NAcM-OPT (2089293-61-6) is a potent and selective NEDD8 ligation inhibitor, IC50=80 nM (in vitro).1? It inhibits the N-terminal acetyl-dependent interaction between the E2 conjugating enzyme UBE2M/UBC12 and DCN1, a subunit of a multiprotein E3 ligase for the ubiquitin-like protein NEDD8, an interaction which is essential for assembly of the multiprotein ligation complex.2 NAcM-OPT inhibits NEDD8 ligation in vitro and in cells (IC50=10 μM) and suppresses anchorage-independent growth of HCC95 cells and is highly selective with respect to other N-terminal acetyl protein binding sites.1 | | Uses | NAcM-OPT inhibits N-Acetyl-mediated interaction between BE2M (E2 conjugating enzyme) and DCN1 (a component of E3 ligase complex). Also has inhibitory action on DCN2 and NEDD8 ubiquitin-like protein. Reduces anchorage-independent growth in DCN1-amplified cell line. | | Definition | ChEBI: 1-Benzyl-1-(1-butyl-4-piperidinyl)-3-(3,4-dichlorophenyl)urea is a member of ureas. | | References | 1) Scott et al. (2017), Blocking an N-terminal acetylation-dependent protein interaction inhibits an E3 ligase; Nat. Chem. Biol., 13 850
2) Scott et al. (2011), N-terminal acetylation acts as an avidity enhancer within an interconnected multiprotein complex; Science, 334 674 |
| | NAcM-OPT Preparation Products And Raw materials |
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