Company Name: |
Sigma-Aldrich
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Tel: |
021-61415566 800-8193336 |
Email: |
orderCN@merckgroup.com |
Products Intro: |
Product Name:Hexanucleotide Mix Purity:Hexanucleotide Mix Package:1EA Remarks:11277081001
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| HEXANUCLEOTIDE MIX Basic information |
| HEXANUCLEOTIDE MIX Chemical Properties |
storage temp. | -20°C | form | solution |
| HEXANUCLEOTIDE MIX Usage And Synthesis |
Uses | Hexanucleotide Mix is a mixture of hexanucleotides of all possible sequences for random-primed DNA labeling. Labeled DNA probes with high specific activity are used in a variety of hybridization techniques:
- Screening of gene libraries
- Southern and northern blots
- In situ hybridizations
- RT-PCR
- Generation of cDNA libraries
- Synthesis of first-strand cDNA
- in the determination of vector titer
- Second strand synthesis
| General Description | Convenient oligonucleotide mixture for rapid random-primed labeling of DNA with radioactive, digoxigenin- or biotin-labeled nucleotides. In this method, the complementary DNA strand is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. |
| HEXANUCLEOTIDE MIX Preparation Products And Raw materials |
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