| Company Name: |
Shanghai Macklin Biochemical Co.,Ltd.
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| Tel: |
15221275939 15221275939 |
| Email: |
shenlinxing@macklin.cn |
| Products Intro: |
Product Name:Chondroitin ABC Lyase
CAS:9024-13-9
Purity:0.3-3?units/mg Package:2U
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| | CHONDROITINASE ABC Basic information |
| Product Name: | CHONDROITINASE ABC | | Synonyms: | CHONDROITINASE ABC;CHONDROITIN ABC LYASE;EC 4.2.2.4;chondroitinase abc F. proteus vulgaris;Chondroitinase ABC from Proteus vulgaris;Chondroitinase ABC, Proteus vulgaris*Not available Japan*;Chondroitinase ABC, Recomb.;chondroitinase-abc bsa free | | CAS: | 9024-13-9 | | MF: | NULL | | MW: | 0 | | EINECS: | 232-777-9 | | Product Categories: | | | Mol File: | Mol File | ![CHONDROITINASE ABC Structure]() |
| | CHONDROITINASE ABC Chemical Properties |
| storage temp. | -20°C | | solubility | 0.01% bovine serum albumin aqueous (BSA) solution: soluble | | form | lyophilized powder | | color | White to off-white |
| | CHONDROITINASE ABC Usage And Synthesis |
| Uses | The enzyme has been used to study the consequences of inducing acute, long-lasting changes in chondroitin sulfate proteoglycans using adult rat brain cells. Glycosaminoglycans have been digested with chondroitinase ABC for chondroitin/dermatan sulfate quantitation in keratocyte cell cultures obtained from fresh bovine corneal stromae. | | Uses | Chondroitinase ABC from Sigma has been used to digest O-linked chondroitin sulfate-like glycosaminoglycan (CSGAG). The enzyme has also been used to digest glycosaminoglycan chains in proteoglycans during the construction and expression of a recombinant versican gene in mammalian systems. Furthermore, it has been used to digest chondroitin sulfate in the proteoglycan complexes. The enzyme has also been used in the removal of glycan chains from aggrecan, which eliminates the axon growth-inhibitory effect of aggrecan. | | Biochem/physiol Actions | Chondroitinase ABC catalyzes the eliminative degradation of polysaccharides containing (1-4)-β-D-hexosaminyl and (1-3)-β-D-glucuronosyl (or (1-3)-α-L-iduronosyl) linkages to disaccharides containing 4-deoxy-β-D-gluc-4-enuronosyl groups. It acts on chondroitin 4-sulfate, chondroitin 6-sulfate, and dermatan sulfate. However, it acts slowly on hyaluronate. The molecular weight is approximately 120 kDa with 2 non-identical subunits of molecular masses 86 kDa and 32 kDa. The pH optimum is 8.0 with chondroitin sulfate and 6.8 with hyaluronic acid. The optimal temperature is 37 °C. 1 mM Zn2+ acts as an inhibitor, while 0.05 M acetate is an activator of the enzyme. |
| | CHONDROITINASE ABC Preparation Products And Raw materials |
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