3-氟-N'-((5-硝基噻吩-2-基)亚甲基)苯甲酰肼
| 中文名称 | 3-氟-N'-((5-硝基噻吩-2-基)亚甲基)苯甲酰肼 |
|---|---|
| 中文同义词 | 3-氟-N'-((5-硝基噻吩-2-基)亚甲基)苯甲酰肼;化合物MITOBLOCK-10;3-氟-N'-((5-硝基噻吩-2-基)亚甲基)苯甲酰肼 |
| 英文名称 | 3-fluoro-N'-[(E)-(5-nitrothiophen-2-yl)methylidene]benzohydrazide |
| 英文同义词 | 3-fluoro-N'-[(E)-(5-nitrothiophen-2-yl)methylidene]benzohydrazide;MitoBloCK-10 (MB-10);Benzoic acid, 3-fluoro-, 2-[(5-nitro-2-thienyl)methylene]hydrazide;3-Fluoro-N'-((5-nitrothiophen-2-yl)methylene)benzohydrazide;MB-10;3-fluoro-N'-[(E)-(5-nitrothiophen-2-yl)m;Mitochondrial,Protein,Tim44,MitoBloCK10,MitoBloCK 10,MitoBloCK-10,inhibit,Inhibitor,Import;3-Fluoro-N'-((5-nitrothiophen-2-yl)methylene)benzohydrazide |
| CAS号 | 394694-98-5 |
| 分子式 | C12H8FN3O3S |
| 分子量 | 293.27 |
| EINECS号 | |
| 相关类别 | |
| Mol文件 | 394694-98-5.mol |
| 结构式 |  |
3-氟-N'-((5-硝基噻吩-2-基)亚甲基)苯甲酰肼 性质
| 密度 | 1.47±0.1 g/cm3(Predicted) |
|---|---|
| 储存条件 | Store at -20°C |
| 酸度系数(pKa) | 11.21±0.46(Predicted) |
| 形态 | 固体 |
| 颜色 | 浅黄至黄色 |
MitoBloCK-10 (MB-10) 是首个减弱 PAM 复合体活性的小分子调节剂。MitoBloCK-10 (MB-10) 可抑制 C 末端结构域的 Tim44 与前体和 Hsp70 结合。
MB-10 (100 μM) is a potential attenuator of protein import into mitochondria.
MB-10 inhibits the import of substrates that use the TIM23 import pathway.
MB-10 (0-100 μM) inhibits protein import into mammalian mitochondria.
Cell Viability Assay
| Cell Line: | A plasmid expressing Su9-Ura3-myc was integrated at the LEU2 locus in WT and tim23-2 strains. |
| Concentration: | 100 μM. |
| Incubation Time: | 30 min. |
| Result: | WT strain expressing Su9-Ura3 failed to grow, whereas the tim23-2 mutant expressing Su9-Ura3 grew much faster. The strains grew at a similar rate when the medium was supplemented with uracil. |
Cell Viability Assay
| Cell Line: | HeLa cells. |
| Concentration: | 0-100 μM. |
| Incubation Time: | 24 h. |
| Result: | Inhibited HeLa cells vibility, with an IC 50 of 17.2 μM. |