| Company Name: |
Sigma-Aldrich
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| Tel: |
021-61415566 800-8193336 |
| Email: |
orderCN@merckgroup.com |
| Products Intro: |
Product Name:Phenol:Chloroform:Isoamyl Alcohol 25:24:1, Saturated with 10mM Tris, pH 8.0, 1mM EDTA
Purity:for molecular biology Package:100mL, 400mL Remarks:P3803
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| Company Name: |
Riedel-de Haen AG
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| Tel: |
800 558-9160 |
| Email: |
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| Products Intro: |
Purity:for molecular biology
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| Company Name: |
SIGMA-RBI
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| Tel: |
800 736 3690 (Orders) |
| Email: |
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| Products Intro: |
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| | Phenol:Chloroform:Isoamyl Alcohol 25:24:1 Saturated with 10mM Tris, pH8.0, 1mM EDTA. Basic information |
| | Phenol:Chloroform:Isoamyl Alcohol 25:24:1 Saturated with 10mM Tris, pH8.0, 1mM EDTA. Chemical Properties |
| | Phenol:Chloroform:Isoamyl Alcohol 25:24:1 Saturated with 10mM Tris, pH8.0, 1mM EDTA. Usage And Synthesis |
| Uses | Removal of proteins from nucleic acids can be achieved by extraction with phenol:chloroform: isoamyl alcohol solutions. For crude mixtures of nucleic acids, digestion with a broad range proteolytic enzyme prior to the phenol:chloroform extraction may be beneficial. Although proteins are efficiently denatured by phenol, RNase activity is not completely inhibited. Therefore, a small amount of isoamyl alcohol is added to further ensure the deactivation of RNase activity. Please see Product Information Sheet for the protocol for removal of proteins from nucleic acids. |
| | Phenol:Chloroform:Isoamyl Alcohol 25:24:1 Saturated with 10mM Tris, pH8.0, 1mM EDTA. Preparation Products And Raw materials |
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